ArticleInvestigative ophthalmology & visual science2010
Ectopic Mitf in the embryonic chick retina by co-transfection of β-catenin and Otx2.
Article in Investigative ophthalmology & visual science, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 35 citations in OpenAlex.
- SWI/SNF complexes are required for retinal pigmented epithelium differentiation and for the inhibition of cell proliferation and neural differentiation programs.Development (Cambridge, England) · 2023Article
- The LHX2-OTX2 transcriptional regulatory module controls retinal pigmented epithelium differentiation and underlies genetic risk for age-related macular degeneration.PLoS biology · 2023Article
- Zfp503/Nlz2 Is Required for RPE Differentiation and Optic Fissure Closure.Investigative ophthalmology & visual science · 2022Article
- Involvement of a Basic Helix-Loop-Helix GeneJournal of developmental biology · 2022Article
- A distal enhancer that directs Otx2 expression in the retinal pigment epithelium and neuroretina.Developmental dynamics : an official publication of the American Association of Anatomists · 2020Article
- Genetics of congenital eye malformations: insights from chick experimental embryology.Human genetics · 2019Review
- Article
- Cellular and Molecular Preconditions for Retinal Pigment Epithelium (RPE) Natural Reprogramming during Retinal Regeneration in Urodela.Biomedicines · 2016Review
- Loss of Axin2 Causes Ocular Defects During Mouse Eye Development.Investigative ophthalmology & visual science · 2016Article
- WNT/β-Catenin Signaling in Vertebrate Eye Development.Frontiers in cell and developmental biology · 2016Review
- Genetic chimeras reveal the autonomy requirements for Vsx2 in embryonic retinal progenitor cells.Neural development · 2015Article
- Wnt ligands from the embryonic surface ectoderm regulate 'bimetallic strip' optic cup morphogenesis in mouse.Development (Cambridge, England) · 2015Article
- Multiple requirements of the focal dermal hypoplasia gene porcupine during ocular morphogenesis.The American journal of pathology · 2015Article
- Loss of MITF expression during human embryonic stem cell differentiation disrupts retinal pigment epithelium development and optic vesicle cell proliferation.Human molecular genetics · 2014Article
- Retinal pigment epithelium development, plasticity, and tissue homeostasis.Experimental eye research · 2014Review
- Article
- Vsx2 controls eye organogenesis and retinal progenitor identity via homeodomain and non-homeodomain residues required for high affinity DNA binding.PLoS genetics · 2012Article
- Compartmentalization of vertebrate optic neuroephithelium: external cues and transcription factors.Molecules and cells · 2012Review
- Article
- Eye morphogenesis and patterning of the optic vesicle.Current topics in developmental biology · 2010Review
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
Abstract
purposeDevelopment of the retinal pigment epithelium (RPE) is controlled by intrinsic and extrinsic regulators including orthodenticle homeobox 2 (Otx2) and the Wnt/β-catenin pathway, respectively. Otx2 and β-catenin are necessary for the expression of the RPE key regulator microphthalmia-associated transcription factor (Mitf); however, neither factor is sufficient to promote Mitf expression in vivo. The study was conducted to determine whether Otx2 and β-catenin act in a combinatorial manner and tested whether co-expression in the presumptive chick retina induces ectopic Mitf expression.
methodsThe sufficiency of Wnt/β-catenin activation and/or Otx2 expression to induce RPE-specific gene expression was examined in chick optic vesicle explant cultures or in the presumptive neural retina using in ovo-electroporation. Luciferase assays were used to examine the transactivation potentials of Otx2 and β-catenin on the Mitf-D enhancer and autoregulation of the Mitf-D and Otx2T0 enhancers.
resultsIn optic vesicles explant cultures, RPE-specific gene expression was activated by lithium chloride, a Wnt/β-catenin agonist. However, in vivo, Mitf was induced only in the presumptive retina if both β-catenin and Otx2 are co-expressed. Furthermore, both Mitf and Otx2 can autoregulate their own enhancers in vitro.
conclusionsThe present study provides evidence that β-catenin and Otx2 are sufficient, at least in part, to convert retinal progenitor cells into presumptive RPE cells expressing Mitf. Otx2 may act as a competence factor that allows RPE specification in concert with additional RPE-promoting factors such as β-catenin.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.