ArticleThe Journal of neuroscience : the official journal of the Society for Neuroscience2010
Alterations of EHD1/EHD4 protein levels interfere with L1/NgCAM endocytosis in neurons and disrupt axonal targeting.
Article in The Journal of neuroscience : the official journal of the Society for Neuroscience, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
27 citing papers in PubMed, 40 citations in OpenAlex.
- A Quarter Century of EHD Protein Research: From Endosomal Recycling to Ciliopathies.Traffic (Copenhagen, Denmark) · 2026Review
- Prediction of genomic biomarkers for endometriosis using the transcriptomic dataset.World journal of clinical cases · 2025Article
- Extracellular vesicle signatures from eye lavage as novel non-invasive biomarkers for hypoxic ischaemic insult-findings from a neonatal mouse model.Frontiers in medical technology · 2025Article
- Identification of drug-like molecules targeting the ATPase activity of dynamin-like EHD4.PloS one · 2024Article
- Article
- Epsin15 Homology Domains: Role in the Pathogenesis of Pulmonary Arterial Hypertension.Frontiers in physiology · 2018Article
- The cellular mechanisms that maintain neuronal polarity.Nature reviews. Neuroscience · 2016Review
- SARA regulates neuronal migration during neocortical development through L1 trafficking.Development (Cambridge, England) · 2016Article
- Exome sequencing for bipolar disorder points to roles of de novo loss-of-function and protein-altering mutations.Molecular psychiatry · 2016Article
- A translational systems biology approach in both animals and humans identifies a functionally related module of accumbal genes involved in the regulation of reward processing and binge drinking in males.Journal of psychiatry & neuroscience : JPN · 2016Article
- Significance of transcytosis in Alzheimer's disease: BACE1 takes the scenic route to axons.BioEssays : news and reviews in molecular, cellular and developmental biology · 2015Article
- Adapting for endocytosis: roles for endocytic sorting adaptors in directing neural development.Frontiers in cellular neuroscience · 2015Review
- Article
- Maturational conversion of dendritic early endosomes and their roles in L1-mediated axon growth.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2014Article
- A function for EHD family proteins in unidirectional retrograde dendritic transport of BACE1 and Alzheimer's disease Aβ production.Cell reports · 2013Article
- X11/Mint genes control polarized localization of axonal membrane proteins in vivo.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2013Article
- Doublecortin (DCX) mediates endocytosis of neurofascin independently of microtubule binding.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2012Article
- Review
- Rabankyrin-5 interacts with EHD1 and Vps26 to regulate endocytic trafficking and retromer function.Traffic (Copenhagen, Denmark) · 2012Article
- Mechanisms of polarized membrane trafficking in neurons -- focusing in on endosomes.Molecular and cellular neurosciences · 2011Review
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
Axon growth is regulated by many proteins, including adhesion molecules, which need to be trafficked correctly to axons. The adhesion molecule L1/neuron-glia cell adhesion molecule (NgCAM) travels to axons via an endocytosis-dependent pathway (transcytosis), traversing somatodendritic endosomes. The Eps15 homology domain (EHD) family proteins (EHD1-EHD4) play important roles in endosomal recycling and possibly in endocytosis. We investigated whether EHD1 regulates L1/NgCAM trafficking in neurons. Both short hairpin-mediated downregulation and overexpression of EHD1 led to dendritic mistargeting of NgCAM. Downregulation of EHD1 showed increased endosomal accumulation of NgCAM, whereas, surprisingly, overexpression of EHD1 led to impairment of L1/NgCAM internalization in neurons but not in fibroblasts. Transferrin internalization, however, was unaffected. At longer overexpression times of EHD1, NgCAM endocytosis returned to normal, suggesting rapid upregulation of compensatory endocytic pathways. EHD1 is capable of hetero-oligomerization, and an endogenous complex of EHD1 and EHD4 was identified previously. We therefore tested whether short-term overexpression of other EHD family members showed a similar endocytosis defect. Expression of EHD4, but not of EHD3, also caused a defect in L1/NgCAM endocytosis. Oligomerization of EHD1 was required to cause NgCAM endocytosis defects, and simultaneous expression of EHD1 and EHD4 rescued NgCAM endocytosis. Therefore, balanced levels of EHD1-EHD4 are important for NgCAM endocytosis in neurons. Our data suggest that EHD1 plays roles in both endosomal recycling and a specialized endocytosis pathway in neurons used by NgCAM. We propose that EHD1 and EHD4 act as hetero-oligomeric complexes in this pathway.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.