ArticleJournal of clinical microbiology2010
Detection of Anaplasma marginale and A. phagocytophilum in bovine peripheral blood samples by duplex real-time reverse transcriptase PCR assay.
Article in Journal of clinical microbiology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 17 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
17 citing papers in PubMed, 41 citations in OpenAlex.
- Comparison of iatrogenic transmission of Anaplasma marginale in Holstein steers via needle and needle-free injection techniques.American journal of veterinary research · 2010Trial
- Myocardial injury associated with naturally occurring clinical bovine anaplasmosis: evaluation of novel cardiac biomarkers.Veterinary research communications · 2026Article
- Application of LAMP and TaqMan qPCR for the rapid diagnosis of Anaplasma Capra (an emerging tick-borne zoonotic pathogen) and comparison with Nested-PCR.Veterinary research communications · 2026Article
- Article
- Immunization withVaccines · 2023Article
- Time and temperature stability ofFrontiers in veterinary science · 2023Article
- Targeted mutagenesis in Anaplasma marginale to define virulence and vaccine development against bovine anaplasmosis.PLoS pathogens · 2022Article
- Recombinase polymerase amplification (RPA) with lateral flow detection for three Anaplasma species of importance to livestock health.Scientific reports · 2021Article
- Epidemiology and genotyping of Anaplasma marginale and co-infection with piroplasms and other Anaplasmataceae in cattle and buffaloes from Egypt.Parasites & vectors · 2020Article
- Transmission Dynamics of Bovine Anaplasmosis in a Cattle Herd.Interdisciplinary perspectives on infectious diseases · 2018Article
- Comparison of three nucleic acid-based tests for detectingThe Onderstepoort journal of veterinary research · 2017Article
- Anaplasma marginale and A. phagocytophilum in cattle in Tunisia.Parasites & vectors · 2016Article
- Important hemoprotozoan diseases of livestock: Challenges in current diagnostics and therapeutics: An update.Veterinary world · 2016Review
- Active surveillance of Anaplasma marginale in populations of arthropod vectors (Acari: Ixodidae; Diptera: Tabanidae) during and after an outbreak of bovine anaplasmosis in southern Manitoba, Canada.Canadian journal of veterinary research = Revue canadienne de recherche veterinaire · 2016Article
- Anaplasma marginale and Anaplasma phagocytophilum: Rickettsiales pathogens of veterinary and public health significance.Parasitology research · 2015Review
- Detection of A. phagocytophilum and E. chaffeensis in patient and mouse blood and ticks by a duplex real-time PCR assay.PloS one · 2013Article
- Sensitivity and specificity of a competitive enzyme-linked immunosorbent assay relative to quantitative reverse transcriptase PCR for detection of Anaplasma marginale and A. phagocytophilum.Journal of clinical microbiology · 2012Article
Corrections and comments
- Commented on by
- Erratum issued
Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Insufficient diagnostic sensitivity and specificity coupled with the potential for cross-reactivity among closely related Anaplasma species has made the accurate determination of infection status problematic. A method for the development of simplex and duplex real-time quantitative reverse transcriptase PCR (qRT-PCR) assays for the detection of A. marginale and A. phagocytophilum 16S rRNA in plasma-free bovine peripheral blood samples is described. The duplex assay was able to detect as few as 100 copies of 16S rRNA of both A. marginale and A. phagocytophilum in the same reaction. The ratio of 16S rRNA to 16S DNA copies for A. marginale was determined to be 117.9:1 (95% confidence interval [95% CI], 100.7:1, 135.2:1). Therefore, the detection limit is the minimum infective unit of one A. marginale bacterium. The duplex assay detected nonequivalent molar ratios as high as 100-fold. Additionally, the duplex assay and a competitive enzyme-linked immunosorbent assay (cELISA) were used to screen 237 samples collected from herds in which anaplasmosis was endemic. When the cELISA was evaluated by the results of the qRT-PCR, its sensitivity and specificity for the detection of A. marginale infection were found to be 65.2% (95% CI, 55.3%, 75.1%) and 97.3% (95% CI, 94.7%, 99.9%), respectively. A. phagocytophilum infection was not detected in the samples analyzed. One- and two-way receiver operator characteristic curves were constructed in order to recommend the optimum negative cutoff value for the cELISA. Percentages of inhibition of 20 and 15.3% were recommended for the one- and two-way curves, respectively. In conclusion, the duplex real-time qRT-PCR assay is a highly sensitive and specific diagnostic tool for the accurate and precise detection of A. marginale and A. phagocytophilum infections in cattle.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.