ArticleThe Journal of biological chemistry2010
Peroxisomal plant 3-ketoacyl-CoA thiolase structure and activity are regulated by a sensitive redox switch.
Article in The Journal of biological chemistry, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 45 citations in OpenAlex.
- The molecular glue CLEO4-88 inhibits the ACAA1 thiolase by induced binding to GID4.Nature chemical biology · 2026Article
- Differential Alteration of Gene Expression by Benzyl Adenine andPlants (Basel, Switzerland) · 2025Article
- Naturally impaired side-chain shortening of aromatic 3-ketoacyl-CoAs reveals the biosynthetic pathway of plant acetophenones.Nature plants · 2025Article
- Multi-Omics Research Accelerates the Clarification of the Formation Mechanism and the Influence of Leaf Color Variation in Tea (Plants (Basel, Switzerland) · 2024Review
- Article
- Harnessing redox proteomics to study metabolic regulation and stress response in lignin-fed Rhodococci.Biotechnology for biofuels and bioproducts · 2023Article
- Peroxisomal KAT2 (3-ketoacyl-CoA thiolase 2) gene has a key role in gingerol biosynthesis in ginger (Journal of plant biochemistry and biotechnology · 2023Article
- Lipid and Protein Oxidation of Brown Rice and Selenium-Rich Brown Rice during Storage.Foods (Basel, Switzerland) · 2022Article
- Interactome of Arabidopsis Thaliana.Plants (Basel, Switzerland) · 2022Article
- Label-Free Proteomic Analysis of Smoke-Drying and Shade-Drying Processes of Postharvest Rhubarb: A Comparative Study.Frontiers in plant science · 2021Article
- The 3-ketoacyl-CoA thiolase: an engineered enzyme for carbon chain elongation of chemical compounds.Applied microbiology and biotechnology · 2020Review
- A glutathione-dependent control of the indole butyric acid pathway supports Arabidopsis root system adaptation to phosphate deprivation.Journal of experimental botany · 2020Article
- Structural basis for differentiation between two classes of thiolase: Degradative vs biosynthetic thiolase.Journal of structural biology: X · 2020Article
- Phaeophyceaean (Brown Algal) Extracts Activate Plant Defense Systems inFrontiers in plant science · 2020Article
- Comparative Proteomic Analysis on Fruit Ripening Processes in Two Varieties of Tropical Mango (Mangifera indica).The protein journal · 2019Article
- Multilevel Regulation of Peroxisomal Proteome by Post-Translational Modifications.International journal of molecular sciences · 2019Review
- Catabolism of the Cholesterol Side Chain in Mycobacterium tuberculosis Is Controlled by a Redox-Sensitive Thiol Switch.ACS infectious diseases · 2017Article
- Developmental transcriptome analysis and identification of genes involved in formation of intestinal air-breathing function of Dojo loach, Misgurnus anguillicaudatus.Scientific reports · 2016Article
- Coenzyme A-free activity, crystal structure, and rational engineering of a promiscuous β-ketoacyl thiolase fromJournal of molecular catalysis. B, Enzymatic · 2015Article
- Redox-switch regulatory mechanism of thiolase from Clostridium acetobutylicum.Nature communications · 2015Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The breakdown of fatty acids, performed by the beta-oxidation cycle, is crucial for plant germination and sustainability. beta-Oxidation involves four enzymatic reactions. The final step, in which a two-carbon unit is cleaved from the fatty acid, is performed by a 3-ketoacyl-CoA thiolase (KAT). The shortened fatty acid may then pass through the cycle again (until reaching acetoacetyl-CoA) or be directed to a different cellular function. Crystal structures of KAT from Arabidopsis thaliana and Helianthus annuus have been solved to 1.5 and 1.8 A resolution, respectively. Their dimeric structures are very similar and exhibit a typical thiolase-like fold; dimer formation and active site conformation appear in an open, active, reduced state. Using an interdisciplinary approach, we confirmed the potential of plant KATs to be regulated by the redox environment in the peroxisome within a physiological range. In addition, co-immunoprecipitation studies suggest an interaction between KAT and the multifunctional protein that is responsible for the preceding two steps in beta-oxidation, which would allow a route for substrate channeling. We suggest a model for this complex based on the bacterial system.
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