ArticleStructure (London, England : 1993)2010
The Prp19 WD40 domain contains a conserved protein interaction region essential for its function.
Article in Structure (London, England : 1993), 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 40 citations in OpenAlex.
- Transcriptomic and targeted metabolomic analysis identifies genes involved in differential anthocyanin accumulation in potato tubers.Frontiers in plant science · 2025Article
- Discovery and Analysis of Repeat and Low-Complexity Architectures in Proteins and Their Conserved Evolutionary Relationships Using Self-Homology Dot Plots.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Article
- The PRP19 Ubiquitin Ligase, Standing at the Cross-Roads of mRNA Processing and Genome Stability.Cancers · 2022Review
- A U-Box Type E3 Ubiquitin Ligase Prp19-Like Protein Negatively Regulates Lipid Accumulation and Cell Size inFrontiers in microbiology · 2022Article
- Structural basis for conformational equilibrium of the catalytic spliceosome.Molecular cell · 2021Article
- The Effect of Mutations in the TPR and Ankyrin Families of Alpha Solenoid Repeat Proteins.Frontiers in bioinformatics · 2021Review
- Topology of the U12-U6ACS omega · 2020Article
- Ubiquitylation at the Fork: Making and Breaking Chains to Complete DNA Replication.International journal of molecular sciences · 2018Review
- Genome-Wide Identification and Characterization of WD40 Protein Genes in the Silkworm, Bombyx mori.International journal of molecular sciences · 2018Article
- MAC3A and MAC3B, Two Core Subunits of the MOS4-Associated Complex, Positively Influence miRNA Biogenesis.The Plant cell · 2018Article
- hPso4/hPrp19: a critical component of DNA repair and DNA damage checkpoint complexes.Oncogene · 2016Review
- CryoEM structures of two spliceosomal complexes: starter and dessert at the spliceosome feast.Current opinion in structural biology · 2016Review
- The NineTeen Complex (NTC) and NTC-associated proteins as targets for spliceosomal ATPase action during pre-mRNA splicing.RNA biology · 2015Article
- Structural and functional insights into the N-terminus of Schizosaccharomyces pombe Cdc5.Biochemistry · 2014Article
- PRP19 transforms into a sensor of RPA-ssDNA after DNA damage and drives ATR activation via a ubiquitin-mediated circuitry.Molecular cell · 2014Article
- Functional roles of protein splicing factors.Bioscience reports · 2012Review
- Crystal structure of Cwc2 reveals a novel architecture of a multipartite RNA-binding protein.The EMBO journal · 2012Article
- Cwc2 and its human homologue RBM22 promote an active conformation of the spliceosome catalytic centre.The EMBO journal · 2012Article
- Systematic two-hybrid and comparative proteomic analyses reveal novel yeast pre-mRNA splicing factors connected to Prp19.PloS one · 2011Article
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Authors and funding
6 authors at 3 institutions in 1 country.
Funding
Abstract
Prp19 is a member of the WD40 repeat family of E3 ubiquitin ligases and a conserved eukaryotic RNA splicing factor essential for activation and stabilization of the spliceosome. To understand the role of the WD40 repeat domain of Prp19 we have determined its structure using X-ray crystallography. The domain has a distorted seven bladed WD40 architecture with significant asymmetry due to irregular packing of blades one and seven into the core of the WD40 domain. Structure-based mutagenesis identified a highly conserved surface centered around blade five that is required for the physical interaction between Prp19 and Cwc2, another essential splicing factor. This region is found to be required for Prp19 function and yeast viability. Experiments in vitro and in vivo demonstrate that two molecules of Cwc2 bind to the Prp19 tetramer. These coupled structural and functional studies provide a model for the functional architecture of Prp19.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.