ArticleBMC genomics2010
Proteomics-based confirmation of protein expression and correction of annotation errors in the Brucella abortus genome.
Article in BMC genomics, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 44 citations in OpenAlex.
- ComparativeVeterinary world · 2026Article
- Genomic and Immunoinformatics Insights Into a Bovine-Derived Brucella abortus S19 Field Strain: Adaptations Impacting Vaccine Efficacy.Veterinary medicine and science · 2025Article
- Article
- Engineering Escherichia coli to Utilize Erythritol as Sole Carbon Source.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2023Article
- Article
- Pan-Proteomic Analysis and Elucidation of Protein Abundance among the Closely RelatedBiomolecules · 2020Article
- Molecular control of gene expression byThe Journal of biological chemistry · 2018Article
- Analysis of protein expression inJournal of veterinary science · 2018Article
- Article
- Relative Quantitative Proteomic Analysis ofFrontiers in microbiology · 2017Article
- Tissue-specific Proteogenomic Analysis of Plutella xylostella Larval Midgut Using a Multialgorithm Pipeline.Molecular & cellular proteomics : MCP · 2016Article
- Immunogenicity and Protective Response Induced by Recombinant Plasmids Based on the BAB1_0267 and BAB1_0270 Open Reading Frames ofFrontiers in cellular and infection microbiology · 2016Article
- The predicted ABC transporter AbcEDCBA is required for type IV secretion system expression and lysosomal evasion by Brucella ovis.PloS one · 2014Article
- Proteogenomic analysis of Bradyrhizobium japonicum USDA110 using GenoSuite, an automated multi-algorithmic pipeline.Molecular & cellular proteomics : MCP · 2013Article
- MLVA16 typing of Portuguese human and animal Brucella melitensis and Brucella abortus isolates.PloS one · 2012Article
- Differential expression of iron acquisition genes by Brucella melitensis and Brucella canis during macrophage infection.PloS one · 2012Article
- The small protein CydX is required for function of cytochrome bd oxidase in Brucella abortus.Frontiers in cellular and infection microbiology · 2012Article
- A proteogenomic analysis of Shigella flexneri using 2D LC-MALDI TOF/TOF.BMC genomics · 2011Article
- Systems biology approach predicts antibody signature associated with Brucella melitensis infection in humans.Journal of proteome research · 2011Article
Corrections and comments
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Authors and funding
9 authors at 3 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundBrucellosis is a major bacterial zoonosis affecting domestic livestock and wild mammals, as well as humans around the globe. While conducting proteomics studies to better understand Brucella abortus virulence, we consolidated the proteomic data collected and compared it to publically available genomic data.
resultsThe proteomic data was compiled from several independent comparative studies of Brucella abortus that used either outer membrane blebs, cytosols, or whole bacteria grown in media, as well as intracellular bacteria recovered at different times following macrophage infection. We identified a total of 621 bacterial proteins that were differentially expressed in a condition-specific manner. For 305 of these proteins we provide the first experimental evidence of their expression. Using a custom-built protein sequence database, we uncovered 7 annotation errors. We provide experimental evidence of expression of 5 genes that were originally annotated as non-expressed pseudogenes, as well as start site annotation errors for 2 other genes.
conclusionsAn essential element for ensuring correct functional studies is the correspondence between reported genome sequences and subsequent proteomics studies. In this study, we have used proteomics evidence to confirm expression of multiple proteins previously considered to be putative, as well as correct annotation errors in the genome of Brucella abortus strain 2308.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.