ArticleMarine biotechnology (New York, N.Y.)2011
Exposure to dsRNA elicits RNA interference in Brachionus manjavacas (Rotifera).
Article in Marine biotechnology (New York, N.Y.), 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
15 citing papers in PubMed.
- A transgenic rotifer-based RNA interference approach for antiviral protection againstFrontiers in microbiology · 2026Article
- A transcriptomic examination of encased rotifer embryos reveals the developmental trajectory leading to long-term dormancy; are they "animal seeds"?BMC genomics · 2024Article
- A genome-wide screening of the 70 kDa heat shock protein (HSP70) genes in the rotifer Brachionus plicatilis sensu stricto with a characterization of two heat-inducible HSP70 genes.Cell stress & chaperones · 2023Article
- RNAi Silencing of the Biomineralization Gene Perlucin Impairs Oyster Ability to Cope with Ocean Acidification.International journal of molecular sciences · 2023Article
- Brachionus rotifers as a model for investigating dietary and metabolic regulators of aging.Nutrition and healthy aging · 2021Review
- Congeneric variability in lifespan extension and onset of senescence suggest active regulation of aging in response to low temperature.Experimental gerontology · 2018Article
- Metagenomic sequencing suggests a diversity of RNA interference-like responses to viruses across multicellular eukaryotes.PLoS genetics · 2018Article
- Moderately lower temperatures greatly extend the lifespan of Brachionus manjavacas (Rotifera): Thermodynamics or gene regulation?Experimental gerontology · 2016Article
- Joint inhibition of TOR and JNK pathways interacts to extend the lifespan of Brachionus manjavacas (Rotifera).Experimental gerontology · 2014Article
- Rotifers as models for the biology of aging.International review of hydrobiology · 2014Article
- Lifespan extension of rotifers by treatment with red algal extracts.Experimental gerontology · 2013Article
- Delivery of nucleic acids, proteins, and nanoparticles by arginine-rich cell-penetrating peptides in rotifers.Marine biotechnology (New York, N.Y.) · 2013Article
- The mate recognition protein gene mediates reproductive isolation and speciation in the Brachionus plicatilis cryptic species complex.BMC evolutionary biology · 2012Article
- Antioxidants can extend lifespan of Brachionus manjavacas (Rotifera), but only in a few combinations.Biogerontology · 2012Article
- Conservation of progesterone hormone function in invertebrate reproduction.Proceedings of the National Academy of Sciences of the United States of America · 2010Article
Corrections and comments
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
RNA interference (RNAi) is a powerful technique for functional genomics, yet no studies have reported its successful application to zooplankton. Many zooplankton, particularly microscopic metazoans of phylum Rotifera, have unique life history traits for which genetic investigation has been limited. In this paper, we report the development of RNAi methods for rotifers, with the exogenous introduction of double-stranded RNA (dsRNA) through the use of a lipofection reagent. Transfection with dsRNA for heat shock protein 90, the membrane-associated progesterone receptor, and mitogen-activated protein kinase significantly increased the proportion of non-reproductive females. Additionally, a fluorescence-based lectin binding assay confirmed the significant suppression of four of six glycosylation enzymes that were targeted with dsRNA. Suppression of mRNA transcripts was confirmed with quantitative PCR. Development of RNAi for rotifers promises to enhance the ability for assessing genetic regulation of features critical to their life history and represents a key step toward functional genomics research in zooplankton.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.