ArticleBritish journal of cancer2010
Image cytometry accurately detects DNA ploidy abnormalities and predicts late relapse to high-grade dysplasia and adenocarcinoma in Barrett's oesophagus following photodynamic therapy.
Article in British journal of cancer, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers, 1 of them a synthesis that pooled it.
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Who cites it
20 citing papers in PubMed, 1 synthesis or guideline pooled it, 53 citations in OpenAlex.
- Genetic Biomarkers of Barrett's Esophagus Susceptibility and Progression to Dysplasia and Cancer: A Systematic Review and Meta-Analysis.Digestive diseases and sciences · 2016Pooled it
- Mixed responses to targeted therapy driven by chromosomal instability through p53 dysfunction and genome doubling.Nature communications · 2024Article
- Genomic Patterns of Malignant Peripheral Nerve Sheath Tumor (MPNST) Evolution Correlate with Clinical Outcome and Are Detectable in Cell-Free DNA.Cancer discovery · 2023Article
- Article
- A biomarker panel predicts progression of Barrett's esophagus to esophageal adenocarcinoma.Diseases of the esophagus : official journal of the International Society for Diseases of the Esophagus · 2019Article
- Quantitation of spatial and temporal variability of biomarkers for Barrett's Esophagus.Diseases of the esophagus : official journal of the International Society for Diseases of the Esophagus · 2017Article
- Robust microbial cell segmentation by optical-phase thresholding with minimal processing requirements.Cytometry. Part A : the journal of the International Society for Analytical Cytology · 2017Article
- Genomics, Endoscopy, and Control of Gastroesophageal Cancers: A Perspective.Cellular and molecular gastroenterology and hepatology · 2017Review
- Surrogate Markers: Lessons from the Next Gen?Cancer prevention research (Philadelphia, Pa.) · 2016Article
- Revisiting tumour aneuploidy - the place of ploidy assessment in the molecular era.Nature reviews. Clinical oncology · 2016Review
- Autofluorescence-Directed Confocal Endomicroscopy in Combination With a Three-Biomarker Panel Can Inform Management Decisions in Barrett's Esophagus.The American journal of gastroenterology · 2015Article
- Whole slide image cytometry: a novel method to detect abnormal DNA content in Barrett's esophagus.Laboratory investigation; a journal of technical methods and pathology · 2015Article
- Genetic Insights in Barrett's Esophagus and Esophageal Adenocarcinoma.Gastroenterology · 2015Review
- Article
- Prediction of response to endoscopic therapy of Barrett's dysplasia by using genetic biomarkers.Gastrointestinal endoscopy · 2014Article
- Biomarkers of Barrett's esophagus.World journal of gastrointestinal pathophysiology · 2014Review
- Barrett's esophagus and cancer risk: how research advances can impact clinical practice.Gut and liver · 2014Review
- Three-dimensional DNA image cytometry by optical projection tomographic microscopy for early cancer diagnosis.Journal of medical imaging (Bellingham, Wash.) · 2014Article
- GERD-Barrett-Adenocarcinoma: Do We Have Suitable Prognostic and Predictive Molecular Markers?Gastroenterology research and practice · 2013Article
- Comparison of nuclear texture analysis and image cytometric DNA analysis for the assessment of dysplasia in Barrett's oesophagus.British journal of cancer · 2011Article
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Authors and funding
11 authors at 4 institutions in 2 countries.
Funding
Abstract
BACKGROUND AND
aimsDNA ploidy abnormalities (aneuploidy/tetraploidy) measured by flow cytometry (FC) are strong predictors of future cancer development in untreated Barrett's oesophagus, independent of histology grade. Image cytometric DNA analysis (ICDA) is an optical technique allowing visualisation of abnormal nuclei that may be undertaken on archival tissue. Our aim was to determine the accuracy of ICDA vs FC, and evaluate DNA ploidy as a prognostic biomarker after histologically successful treatment with photodynamic therapy (PDT).
methodsNuclei were extracted from 40 mum sections of paraffin-embedded biopsies and processed for ICDA at UCL and FC at UW using standardised protocols. Subsequently, DNA ploidy was evaluated by ICDA on a cohort of 30 patients clear of dysplasia 1 year after aminolaevulinic acid PDT for high-grade dysplasia (HGD). The results were correlated with long-term outcome.
resultsIn the comparative study, 93% (41 out of 44) of cases were classified identically. Errors occurred in the near-diploid region by ICDA and the tetraploid region by FC. In the cohort study, there were 13 cases of late relapse (7 cancer, 6 HGD) and 17 patients who remained free of dysplasia after a mean follow-up of 44 months. Aneuploidy post-PDT was highly predictive for recurrent HGD or cancer with a hazard ratio of 8.2 (1.8-37.8) (log-rank P=0.001).
conclusionsICDA is accurate for the detection of DNA ploidy abnormalities when compared with FC. After histologically successful PDT, patients with residual aneuploidy are significantly more likely to develop HGD or cancer than those who become diploid. DNA ploidy by ICDA is a valuable prognostic biomarker after ablative therapy.
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