ArticleDevelopment (Cambridge, England)2010
The BMP pathway acts to directly regulate Tbx20 in the developing heart.
Article in Development (Cambridge, England), 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
39 citing papers in PubMed, 52 citations in OpenAlex.
- Hox/Meis-dependent gene-regulatory transition underlies cardiopharyngeal neural crest diversification.The EMBO journal · 2026Article
- The Data Distillery: A Graph Framework for Semantic Integration and Querying of Biomedical Data.bioRxiv : the preprint server for biology · 2025Article
- Single-nuclei multiomics analysis identifies abnormal cardiomyocytes in a murine model of cardiac development.Nature communications · 2025Article
- Chromosomal Location and Identification ofDiagnostics (Basel, Switzerland) · 2025Article
- Disparity of gene expression in coronary artery disease: insights from MEIS1, HIRA, and Myocardin.Molecular biology reports · 2024Article
- Genetic and functional variants of the TBX20 gene promoter in dilated cardiomyopathy.Molecular genetics & genomic medicine · 2024Article
- SMAD1 Loss-of-Function Variant Responsible for Congenital Heart Disease.BioMed research international · 2022Article
- The Role ofFrontiers in cell and developmental biology · 2021Review
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- CHDGKB: a knowledgebase for systematic understanding of genetic variations associated with non-syndromic congenital heart disease.Database : the journal of biological databases and curation · 2020Article
- Article
- Xenbase: Facilitating the Use ofFrontiers in physiology · 2019Review
- Xenopus as a model organism for birth defects-Congenital heart disease and heterotaxy.Seminars in cell & developmental biology · 2016Review
- Signaling Pathways and Gene Regulatory Networks in Cardiomyocyte Differentiation.Tissue engineering. Part B, Reviews · 2015Review
- Article
- Rac1 signaling is critical to cardiomyocyte polarity and embryonic heart development.Journal of the American Heart Association · 2014Article
- Differential regulation of CASZ1 protein expression during cardiac and skeletal muscle development.Developmental dynamics : an official publication of the American Association of Anatomists · 2014Article
- Proteomic profiling of cardiac tissue by isolation of nuclei tagged in specific cell types (INTACT).Development (Cambridge, England) · 2014Article
- A Gro/TLE-NuRD corepressor complex facilitates Tbx20-dependent transcriptional repression.Journal of proteome research · 2013Article
- 3-OST-7 regulates BMP-dependent cardiac contraction.PLoS biology · 2013Article
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
Abstract
TBX20 has been shown to be essential for vertebrate heart development. Mutations within the TBX20 coding region are associated with human congenital heart disease, and the loss of Tbx20 in a wide variety of model systems leads to cardiac defects and eventually heart failure. Despite the crucial role of TBX20 in a range of cardiac cellular processes, the signal transduction pathways that act upstream of Tbx20 remain unknown. Here, we have identified and characterized a conserved 334 bp Tbx20 cardiac regulatory element that is directly activated by the BMP/SMAD1 signaling pathway. We demonstrate that this element is both necessary and sufficient to drive cardiac-specific expression of Tbx20 in Xenopus, and that blocking SMAD1 signaling in vivo specifically abolishes transcription of Tbx20, but not that of other cardiac factors, such as Tbx5 and MHC, in the developing heart. We further demonstrate that activation of Tbx20 by SMAD1 is mediated by a set of novel, non-canonical, high-affinity SMAD-binding sites located within this regulatory element and that phospho-SMAD1 directly binds a non-canonical SMAD1 site in vivo. Finally, we show that these non-canonical sites are necessary and sufficient for Tbx20 expression in Xenopus, and that reporter constructs containing these sites are expressed in a cardiac-specific manner in zebrafish and mouse. Collectively, our findings define Tbx20 as a direct transcriptional target of the BMP/SMAD1 signaling pathway during cardiac maturation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.