ArticleBMC infectious diseases2010
Rapid semi-automated quantitative multiplex tandem PCR (MT-PCR) assays for the differential diagnosis of influenza-like illness.
Article in BMC infectious diseases, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 41 citations in OpenAlex.
- Trial
- Comparison of MT-PCR with Quantitative PCR for Human Bocavirus in Respiratory Samples with Multiple Respiratory Viruses Detection.Diagnostics (Basel, Switzerland) · 2023Article
- Clinical Evaluation of the cobas SARS-CoV-2 Test and a Diagnostic Platform Switch during 48 Hours in the Midst of the COVID-19 Pandemic.Journal of clinical microbiology · 2020Article
- Hemagglutinin and Neuraminidase Antibodies Are Induced in an Age- and Subtype-Dependent Manner after Influenza Virus Infection.Journal of virology · 2020Article
- The health and economic burden of respiratory syncytial virus associated hospitalizations in adults.PloS one · 2020Article
- Respiratory syncytial virus hospitalisations among young children: a data linkage study.Epidemiology and infection · 2019Article
- Respiratory virus detection and co-infection in children and adults in a large Australian hospital in 2009-2015.Journal of paediatrics and child health · 2018Observational
- Interactive effects of age and respiratory virus on severe lower respiratory infection.Epidemiology and infection · 2018Article
- A multiplex liquid-chip assay based on Luminex xMAP technology for simultaneous detection of six common respiratory viruses.Oncotarget · 2017Article
- Accurate Detection of Avian Respiratory Viruses by Use of Multiplex PCR-Based Luminex Suspension Microarray Assay.Journal of clinical microbiology · 2016Article
- Molecular diagnosis of respiratory viruses.Pathology · 2015Review
- Semiquantitative multiplexed tandem PCR for detection and differentiation of four Theileria orientalis genotypes in cattle.Journal of clinical microbiology · 2015Article
- Effectiveness of seasonal trivalent inactivated influenza vaccine in preventing influenza hospitalisations and primary care visits in Auckland, New Zealand, in 2013.Euro surveillance : bulletin Europeen sur les maladies transmissibles = European communicable disease bulletin · 2014Article
- Article
- Outcomes of influenza A(H1N1)pdm09 virus infection: results from two international cohort studies.PloS one · 2014Article
- Comparison of a multiplex real-time PCR assay with a multiplex Luminex assay for influenza virus detection.Journal of clinical microbiology · 2013Article
- The impact of bacterial and viral co-infection in severe influenza.Influenza and other respiratory viruses · 2013Article
- Evaluation of the Cepheid Xpert Flu Assay for rapid identification and differentiation of influenza A, influenza A 2009 H1N1, and influenza B viruses.Journal of clinical microbiology · 2012Article
- Implementing hospital-based surveillance for severe acute respiratory infections caused by influenza and other respiratory pathogens in New Zealand.Western Pacific surveillance and response journal : WPSARArticle
Corrections and comments
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Authors and funding
8 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundInfluenza A, including avian influenza, is a major public health threat in developed and developing countries. Rapid and accurate detection is a key component of strategies to contain spread of infection, and the efficient diagnosis of influenza-like-illness is essential to protect health infrastructure in the event of a major influenza outbreak.
methodsWe developed a multiplexed PCR (MT-PCR) assay for the simultaneous diagnosis of respiratory viruses causing influenza-like illness, including the specific recognition of influenza A haemagglutinin subtypes H1, H3, and H5. We tested several hundred clinical specimens in two diagnostic reference laboratories and compared the results with standard techniques.
resultsThe sensitivity and specificity of these assays was higher than individual assays based on direct antigen detection and standard PCR against a range of control templates and in several hundred clinical specimens. The MT-PCR assays provided differential diagnoses as well as potentially useful quantitation of virus in clinical samples.
conclusionsMT-PCR is a potentially powerful tool for the differential diagnosis of influenza-like illness in the clinical diagnostic laboratory.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.