ArticleJournal of cellular physiology2010
Centrosome biogenesis continues in the absence of microtubules during prolonged S-phase arrest.
Article in Journal of cellular physiology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
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Who cites it
8 citing papers in PubMed, 11 citations in OpenAlex.
- Review
- Centriolar satellite- and hMsd1/SSX2IP-dependent microtubule anchoring is critical for centriole assembly.Molecular biology of the cell · 2015Article
- One to only two: a short history of the centrosome and its duplication.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2014Review
- Such small hands: the roles of centrins/caltractins in the centriole and in genome maintenance.Cellular and molecular life sciences : CMLS · 2012Review
- Cortical cytasters: a highly conserved developmental trait of Bilateria with similarities to Ctenophora.EvoDevo · 2011Article
- Centrosome amplification in CHO and DT40 cells by inactivation of cyclin-dependent kinases.Cytoskeleton (Hoboken, N.J.) · 2011Article
- Apparent diffusive motion of centrin foci in living cells: implications for diffusion-based motion in centriole duplication.Physical biology · 2011Article
- Centriole duplication: analogue control in a digital age.Cell biology international · 2010Review
Corrections and comments
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Authors and funding
8 authors at 3 institutions in 2 countries.
Funding
Abstract
When CHO cells are arrested in S-phase, they undergo repeated rounds of centrosome duplication without cell-cycle progression. While the increase is slow and asynchronous, the number of centrosomes in these cells does rise with time. To investigate mechanisms controlling this duplication, we have arrested CHO cells in S-phase for up to 72 h, and coordinately inhibited new centriole formation by treatment with the microtubule poison colcemid. We find that in such cells, the pre-existing centrosomes remain, and a variable number of foci--containing alpha/gamma-tubulin and centrin 2--assemble at the nuclear periphery. When the colcemid is washed out, the nuclear-associated foci disappear, and cells assemble new centriole-containing centrosomes, which accumulate the centriole scaffold protein SAS-6, nucleate microtubule asters, and form functional mitotic spindle poles. The number of centrosomes that assemble following colcemid washout increases with duration of S-phase arrest, even though the number of nuclear-associated foci or pre-existing centrosomes does not increase. This suggests that during S-phase, a cryptic generative event occurs repeatedly, even in the absence of new triplet microtubule assembly. When triplet microtubule assembly is restored, these cryptic generative events become realized, and multiple centriole-containing centrosomes assemble.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.