ArticleEuropean biophysics journal : EBJ2010
Sieving experiments and pore diameter: it's not a simple relationship.
Article in European biophysics journal : EBJ, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
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Who cites it
5 citing papers in PubMed.
- Interacting ions in biophysics: real is not ideal.Biophysical journal · 2013Review
- Voltage-gated proton channels: molecular biology, physiology, and pathophysiology of the H(V) family.Physiological reviews · 2013Review
- Current and selectivity in a model sodium channel under physiological conditions: Dynamic Monte Carlo simulations.Biochimica et biophysica acta · 2012Article
- Selectivity sequences in a model calcium channel: role of electrostatic field strength.European biophysics journal : EBJ · 2011Article
- Simulations of calcium channel block by trivalent cations: Gd(3+) competes with permeant ions for the selectivity filter.Biochimica et biophysica acta · 2010Article
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2 authors.
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Abstract
The classic sieving experiment for estimating an ion channel's diameter with successively larger ions is re-examined. Using a very reduced model of a calcium channel, it is shown that sieving experiments measure a combination of three mechanisms: the cross-sectional area available to the sieving ions (the classic interpretation), the exclusion of the sieving ions from a pore crowded with amino acid side chains that protrude into the permeation pathway, and competitive selectivity of the sieving ions with other ions in the bath (even if those are present only at trace concentrations). The latter two can be called sieving-by-crowding because they stem from the excluded volume of the amino acids in the permeation pathway. The model shows that--to a first--order approximation-sieving experiments measure the available volume inside a selectivity filter, rather than the available cross-sectional area. The two are only the same if the narrow part of the pore does not have flexible amino acid side chains interacting directly with the permeant ions; this may be true of potassium channels, but not calcium, sodium, and other channels with "crowded" selectivity filters.
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