ArticleNucleic acids research2010
Multiple roles of the RNA polymerase {beta}' SW2 region in transcription initiation, promoter escape, and RNA elongation.
Article in Nucleic acids research, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
19 citing papers in PubMed, 28 citations in OpenAlex.
- RapA opens the RNA polymerase clamp to disrupt post-termination complexes and prevent cytotoxic R-loop formation.Nature structural & molecular biology · 2025Article
- RNA polymerase pausing, stalling and bypass during transcription of damaged DNA: from molecular basis to functional consequences.Nucleic acids research · 2022Review
- Region 3.2 of the σ factor controls the stability of rRNA promoter complexes and potentiates their repression by DksA.Nucleic acids research · 2018Article
- The RNA polymerase clamp interconverts dynamically among three states and is stabilized in a partly closed state by ppGpp.Nucleic acids research · 2018Article
- Source of the Fitness Defect in Rifamycin-Resistant Mycobacterium tuberculosis RNA Polymerase and the Mechanism of Compensation by Mutations in the β' Subunit.Antimicrobial agents and chemotherapy · 2018Article
- A Thermus phage protein inhibits host RNA polymerase by preventing template DNA strand loading during open promoter complex formation.Nucleic acids research · 2018Article
- RNA polymerase gate loop guides the nontemplate DNA strand in transcription complexes.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- Coupling of downstream RNA polymerase-promoter interactions with formation of catalytically competent transcription initiation complex.Journal of molecular biology · 2014Article
- Structural basis of transcription initiation by bacterial RNA polymerase holoenzyme.The Journal of biological chemistry · 2014Article
- Distinct functions of the RNA polymerase σ subunit region 3.2 in RNA priming and promoter escape.Nucleic acids research · 2014Article
- Structural basis for promoter specificity switching of RNA polymerase by a phage factor.Genes & development · 2014Article
- The σ70 region 1.2 regulates promoter escape by unwinding DNA downstream of the transcription start site.Nucleic acids research · 2013Article
- Frequency, spectrum, and nonzero fitness costs of resistance to myxopyronin in Staphylococcus aureus.Antimicrobial agents and chemotherapy · 2012Article
- A dual switch controls bacterial enhancer-dependent transcription.Nucleic acids research · 2012Article
- Distinct functions of regions 1.1 and 1.2 of RNA polymerase σ subunits from Escherichia coli and Thermus aquaticus in transcription initiation.The Journal of biological chemistry · 2012Article
- Structural transitions in the transcription elongation complexes of bacterial RNA polymerase during σ-dependent pausing.Nucleic acids research · 2012Article
- New target for inhibition of bacterial RNA polymerase: 'switch region'.Current opinion in microbiology · 2011Review
- Antibiotics trapping transcription initiation intermediates: To melt or to bend, what's first?Transcription · 2011Article
- Region 4 of the RNA polymerase σ subunit counteracts pausing during initial transcription.The Journal of biological chemistryArticle
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
Abstract
Interactions of RNA polymerase (RNAP) with nucleic acids must be tightly controlled to ensure precise and processive RNA synthesis. The RNAP β'-subunit Switch-2 (SW2) region is part of a protein network that connects the clamp domain with the RNAP body and mediates opening and closing of the active center cleft. SW2 interacts with the template DNA near the RNAP active center and is a target for antibiotics that block DNA melting during initiation. Here, we show that substitutions of a conserved Arg339 residue in the Escherichia coli RNAP SW2 confer diverse effects on transcription that include defects in DNA melting in promoter complexes, decreased stability of RNAP/promoter complexes, increased apparent K(M) for initiating nucleotide substrates (2- to 13-fold for different substitutions), decreased efficiency of promoter escape, and decreased stability of elongation complexes. We propose that interactions of Arg339 with DNA directly stabilize transcription complexes to promote stable closure of the clamp domain around nucleic acids. During initiation, SW2 may cooperate with the σ(3.2) region to stabilize the template DNA strand in the RNAP active site. Together, our data suggest that SW2 may serve as a key regulatory element that affects transcription initiation and RNAP processivity through controlling RNAP/DNA template interactions.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.