ArticleThe Journal of biological chemistry2010
A novel plant vacuolar Na+/H+ antiporter gene evolved by DNA shuffling confers improved salt tolerance in yeast.
Article in The Journal of biological chemistry, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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10 citing papers in PubMed, 28 citations in OpenAlex.
- Structure-Guided Identification of Critical Residues in the Vacuolar Cation/Proton Antiporter NHX1 fromPlants (Basel, Switzerland) · 2023Article
- Improving Thermostability of Chimeric Enzymes Generated by Domain Shuffling Between Two Different Original Glucoamylases.Frontiers in bioengineering and biotechnology · 2022Article
- Toxic trace element resistance genes and systems identified using the shotgun metagenomics approach in an Iranian mine soil.Environmental science and pollution research international · 2021Article
- Transcriptome-IPMS analysis reveals a tissue-dependent miR156/SPL13 regulatory mechanism in alfalfa drought tolerance.BMC genomics · 2020Article
- New Insights into the Structure-Function Relationship of the Endosomal-Type NaInternational journal of molecular sciences · 2020Article
- Review
- Two NHX-type transporters from Helianthus tuberosus improve the tolerance of rice to salinity and nutrient deficiency stress.Plant biotechnology journal · 2018Article
- Hydrophilic C terminus of Salicornia europaea vacuolar Na(+)/H(+) antiporter is necessary for its function.Journal of genetics · 2014Article
- Cloning and characterization of a novel vacuolar Na+/H+ antiporter gene (Dgnhx1) from chrysanthemum.PloS one · 2013Article
- Vacuolar H+-ATPase works in parallel with the HOG pathway to adapt Saccharomyces cerevisiae cells to osmotic stress.Eukaryotic cell · 2012Article
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Authors and funding
4 authors at 2 institutions in 2 countries.
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No grant is acknowledged in the PubMed record.
Abstract
Plant vacuolar Na(+)/H(+) antiporters play important roles in maintaining cellular ion homeostasis and mediating the transport of Na(+) out of the cytosol and into the vacuole. Vacuolar antiporters have been shown to play significant roles in salt tolerance; however the relatively low V(max) of the Na(+)/H(+) exchange of the Na(+)/H(+) antiporters identified could limit its application in the molecular breeding of salt tolerant crops. In this study, we applied DNA shuffling methodology to generate and recombine the mutations of Arabidopsis thaliana vacuolar Na(+)/H(+) antiporter gene AtNHX1. Screening using a large scale yeast complementation system identified AtNHXS1, a novel Na(+)/H(+) antiporter. Expression of AtNHXS1 in yeast showed that the antiporter localized to the vacuolar membrane and that its expression improved the tolerance of yeast to NaCl, KCl, LiCl, and hygromycin B. Measurements of the ion transport activity across the intact yeast vacuole demonstrated that the AtNHXS1 protein showed higher Na(+)/H(+) exchange activity and a slightly improved K(+)/H(+) exchange activity.
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