ArticleJournal of anatomy2010
Characterization of eight different tetracyclines: advances in fluorescence bone labeling.
Article in Journal of anatomy, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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Who cites it
33 citing papers in PubMed.
- The detection of oral pre- malignant lesions with an autofluorescence based imaging system (VELscope™) - a single blinded clinical evaluation.Head & face medicine · 2013Trial
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- Ultra-thin porous calcium phosphate coatings show extensive remodeling and high osseointegration after 24 weeks in a rabbit femoral condyle model.Frontiers in bioengineering and biotechnology · 2026Article
- Use of digital image analysis to improve rigor and efficiency of physeal bone growth measurements.Histology and histopathology · 2025Article
- Tissue-seeking dyes for in vivo applications.Smart molecules : open access · 2024Review
- Transcription Attenuation in Synthetic Promoters in Nonoverlapping Tandem Formation.Biochemistry · 2024Article
- Decoupling the Effects of Collagen Alignment and Bioceramic Incorporation on Osteoblast Proliferation, Differentiation, and Mineralization.Materials today. Communications · 2024Article
- The Physiological Inorganic Polymers Biosilica and Polyphosphate as Key Drivers for Biomedical Materials in Regenerative Nanomedicine.International journal of nanomedicine · 2024Review
- Advances in Intraoperative Glioma Tissue Sampling and Infiltration Assessment.Brain sciences · 2023Review
- Enhancement of Scaffold In Vivo Biodegradability for Bone Regeneration Using P28 Peptide Formulations.Pharmaceuticals (Basel, Switzerland) · 2023Article
- Simultaneous Labeling of Adipogenic and Osteogenic Differentiating Stem Cells for Live Confocal Analysis.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Effects of Low Intensity Pulsed Ultrasound Stimulation on the Temporal Dynamics of Irradiated Bone Tissue Healing: A Histomorphometric Study in Rabbits.International journal of molecular sciences · 2022Article
- Inhibition of the HIF-1 Survival Pathway as a Strategy to Augment Photodynamic Therapy Efficacy.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Interest of Bone Histomorphometry in Bone Pathophysiology Investigation: Foundation, Present, and Future.Frontiers in endocrinology · 2022Review
- Fluorescence-Guided Surgery in the Surgical Treatment of Gliomas: Past, Present and Future.Cancers · 2021Review
- Structural Similarities between Some Common Fluorophores Used in Biology, Marketed Drugs, Endogenous Metabolites, and Natural Products.Marine drugs · 2020Article
- Investigation of Bone Growth in Additive-Manufactured Pedicle Screw Implant by Using Ti-6Al-4V and Bioactive Glass Powder Composite.International journal of molecular sciences · 2020Article
- Tensile force-induced PDGF-BB/PDGFRβ signals in periodontal ligament fibroblasts activate JAK2/STAT3 for orthodontic tooth movement.Scientific reports · 2020Article
- Lymphatics in bone arise from pre-existing lymphatics.Development (Cambridge, England) · 2020Article
- Scalable MSC-derived bone tissue modules: In vitro assessment of differentiation, matrix deposition, and compressive load bearing.Acta biomaterialia · 2019Article
Corrections and comments
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Authors and funding
10 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Polychrome sequential labeling with fluorochromes is a standard technique for the investigation of bone formation and regeneration processes in vivo. However, for human application, only tetracycline and its derivates are approved as fluorochromes. Therefore, the aim of this study was to determine the fluorescence characteristics of the different tetracycline derivates to assess the feasibility of sequential in vivo bone labeling using distinguishable fluorochromes. Eight different tetracycline derivates were injected subcutaneously into growing rats as a single dose or sequentially in different combinations. After preparation of resin-embedded undecalcified bone sections, the fluorescence properties of the tetracycline derivates in bone were analyzed using conventional fluorescence microscopy, spectral image analysis and confocal laser scanning microscopy. Each tetracycline derivate exhibited a characteristic fluorescence spectrum, but the differences between them were small. Chlortetracycline could be discriminated reliably from all other derivates and could therefore be combined with any other tetracycline derivate for reliably distinguishable double labeling. Tetracycline itself exhibited the brightest fluorescence of all the investigated derivates. Interestingly, in conventional microscopy the same tetracycline derivative can appear in different colours to the human eye, even if spectral analysis confirmed identical emission peaks. In conclusion, the data suggest that fluorescence double labeling of bone is feasible using appropriate tetracycline derivates in combination with spectral imaging modalities.
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