Evidence map›Paper›PMID 20456509›Full record

ArticleInsect molecular biology2010

Validation of novel promoter sequences derived from two endogenous ubiquitin genes in transgenic Aedes aegypti.

M A E Anderson, T L Gross, K M Myles, Z N Adelman

Abstract read
In one paragraph

Article in Insect molecular biology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 70 papers.

0numbers the graph read from it
0cells of the map it votes in
70citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

70 citing papers in PubMed.

  1. Article
  2. Article
  3. Article
  4. Article
  5. Differential elimination of marked sex chromosomes enables production of nontransgenic male mosquitoes in a single strain.Proceedings of the National Academy of Sciences of the United States of America · 2025
    Article
  6. Article
  7. The PAZ domain ofRNA (New York, N.Y.) · 2025
    Article
  8. Article
  9. History of Mosquito Transgenesis: A Perspective in Review.Methods in molecular biology (Clifton, N.J.) · 2025
    Review
  10. Article
  11. Article
  12. Article
  13. Validation of a heat-induciblebioRxiv : the preprint server for biology · 2023
    Article
  14. Article
  15. ImprovedInternational journal of molecular sciences · 2023
    Article
  16. Mimicking superinfection exclusion disrupts alphavirus infection and transmission in the yellow fever mosquitoProceedings of the National Academy of Sciences of the United States of America · 2023
    Article
  17. Article
  18. Article
  19. Article
  20. Article

10 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

M A E AndersonFralin Life Science Institute and Department of Entomology, Virginia Polytechnic Institute and State University, Blacksburg, VA 24061, USA.
T L Gross
K M Myles
Z N Adelman

Funding

Antiviral Pathways in Disease Vector MosquitoesR01AI085091 · NIAID · VIRGINIA POLYTECHNIC INST AND ST UNIV · PI ADELMAN, ZACH N. · 2010 to 2014
$1.9M
Selfish gene and genetic control of vector-borne diseasesR21AI071208 · NIAID · VIRGINIA POLYTECHNIC INST AND ST UNIV · PI ADELMAN, ZACH N. · 2007 to 2008
$403k
NIAID NIH HHS 1R21AI071208-01A1NIAID NIH HHS R01 AI085091NIAID NIH HHS R21 AI071208
6 · The paper itself

Abstract

To date, only a limited number of promoter sequences have been described to drive transgene expression in the disease vector Aedes aegypti. We sought to increase this repertoire by characterizing the ability of upstream sequences derived from the Ae. aegypti Ub(L40) and polyubiquitin genes to drive the expression of marker proteins. Both genomic fragments were able to drive robust expression of luciferase in cultured mosquito cells. Following Mos1-transformation, the Ub(L40) promoter drove strong expression of a fluorescent marker in early larvae and in ovaries, while the polyubiquitin promoter drove robust EGFP expression in all stages of development, including constitutive expression throughout the midgut. These promoter fragments provide two new expression profiles for future Ae. aegypti genetic experiments.

Indexed as

AedesAnimalsAnimals, Genetically ModifiedBase SequenceGene Expression RegulationGenes, InsectGenes, ReporterGreen Fluorescent ProteinsPolyubiquitinPromoter Regions, GeneticReproducibility of ResultsTransformation, GeneticUbiquitinenhanced green fluorescent proteinGreen Fluorescent ProteinsPolyubiquitinUbiquitin

Identifiers

PMID20456509
PMCPMC3605713

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.