ArticleFEMS microbiology letters2010
Comparison of expression vectors in Lactobacillus reuteri strains.
Article in FEMS microbiology letters, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 53 citations in OpenAlex.
- Harnessing CRISPR-Cas9 for Lactobacillus improvement in silage production: current knowledge and future perspectives.Journal of animal science and biotechnology · 2025Review
- Escherichia coli gene expression is influenced more by gut environmental changes from inflammation and microbiota modulation than by colitis severity.Scientific reports · 2025Article
- Function and contribution of two putativeInfection and immunity · 2024Article
- Brussowvirus SW13 Requires a Cell Surface-Associated Polysaccharide To Recognize Its Streptococcus thermophilus Host.Applied and environmental microbiology · 2022Article
- Features and Colonization Strategies ofFrontiers in microbiology · 2022Article
- Angiotensin-(1-7) Expressed From Lactobacillus Bacteria Protect Diabetic Retina in Mice.Translational vision science & technology · 2020Article
- Peptide-Mediated Gene Transfer into Marine Purple Photosynthetic Bacteria.International journal of molecular sciences · 2020Article
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- Challenges and Pitfalls in the Engineering of Human Interleukin 22 (hIL-22) SecretingFrontiers in bioengineering and biotechnology · 2020Article
- Expression of Human ACE2 in Lactobacillus and Beneficial Effects in Diabetic Retinopathy in Mice.Molecular therapy. Methods & clinical development · 2019Article
- Blocking HIV-1 Infection by Chromosomal Integrative Expression of Human CD4 on the Surface of Lactobacillus acidophilus ATCC 4356.Journal of virology · 2019Article
- Broad range shuttle vector construction and promoter evaluation for the use ofSynthetic biology (Oxford, England) · 2019Article
- Characterization of aBiotechnology reports (Amsterdam, Netherlands) · 2018Article
- Engineering Components of theFrontiers in microbiology · 2018Review
- A flow-cytometric method to evaluate eosinophil-mediated uptake of probiotic Lactobacillus reuteri.Journal of microbiological methods · 2017Article
- Article
- Immortalized MH-S cells lack defining features of primary alveolar macrophages and do not support mouse pneumovirus replication.Immunology letters · 2016Article
- Colonization of the Intestinal Tract of the Polyphagous Pest Spodoptera littoralis with the GFP-Tagged Indigenous Gut Bacterium Enterococcus mundtii.Frontiers in microbiology · 2016Article
- Lactobacillus surface layer proteins: structure, function and applications.Applied microbiology and biotechnology · 2013Review
- Distribution dynamics of recombinant Lactobacillus in the gastrointestinal tract of neonatal rats.PloS one · 2013Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The synthesis of heterologous proteins in lactobacilli is strongly influenced by the promoter selected for the expression. In addition, the activity of the promoters themselves may vary among different bacterial hosts. Three different promoters were investigated for their capability to drive enhanced green fluorescent protein (EGFP) expression in Lactococcus lactis spp. cremoris MG1363, in Lactobacillus reuteri DSM 20016(T) and in five L. reuteri strains isolated from chicken crops. The promoters of the Lactobacillus acidophilus surface layer protein gene (slp), L. acidophilus lactate dehydrogenase gene (ldhL) and enterococcal rRNA adenine N-6-methyltransferase gene (ermB) were fused to the coding sequence of EGFP and inserted into the backbone of the pTRKH3 shuttle vector (pTRKH3-slpGFP, pTRKH3-ldhGFP, pTRKH3-ermGFP). Besides conventional analytical methods, a new quick fluorimetric approach was set up to quantify the EGFP fluorescence in transformed clones using the Qubit() fluorometer. ermB proved to be the most effective promoter in L. reuteri isolates, producing 3.90 x 10(-7) g of fluorescent EGFP (mL OD(stationary culture))(-1). Under the same conditions, the ldhL promoter produced 2.66 x 10(-7) g of fluorescent EGFP (mL OD(stationary culture))(-1). Even though the slp promoter was efficient in L. lactis spp. cremoris MG1363, it was nearly inactive both in L. reuteri DSM 20016(T) and in L. reuteri isolates.
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