ArticlePhysical chemistry chemical physics : PCCP2010
Resolution and polarization distribution in cryogenic DNP/MAS experiments.
Article in Physical chemistry chemical physics : PCCP, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.
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Who cites it
38 citing papers in PubMed.
- Investigation of biomolecular dynamics by sensitivity-enhancedJournal of magnetic resonance open · 2024Article
- Resolution in cryogenic solid state NMR: Challenges and solutions.Protein science : a publication of the Protein Society · 2024Review
- A high-field cellular DNP-supported solid-state NMR approach to study proteins with sub-cellular specificity.Chemical science · 2023Article
- Polarizing agents for efficient high field DNP solid-state NMR spectroscopy under magic-angle spinning: from design principles to formulation strategies.Chemical science · 2023Review
- Magic angle spinning NMR of G protein-coupled receptors.Progress in nuclear magnetic resonance spectroscopy · 2022Review
- Time domain DNP at 1.2 T.Journal of magnetic resonance (San Diego, Calif. : 1997) · 2021Article
- Dynamics in Flexible Pillar[The journal of physical chemistry. C, Nanomaterials and interfaces · 2021Article
- A biradical-tagged phospholipid as a polarizing agent for solid-state MAS Dynamic Nuclear Polarization NMR of membrane proteins.Solid state nuclear magnetic resonance · 2019Article
- Article
- Maximizing NMR signal per unit time by facilitating the e-e-n cross effect DNP rate.Physical chemistry chemical physics : PCCP · 2018Article
- Electron decoupling with cross polarization and dynamic nuclear polarization below 6 K.Journal of magnetic resonance (San Diego, Calif. : 1997) · 2018Article
- Pulsed Electron Decoupling and Strategies for Time Domain Dynamic Nuclear Polarization with Magic Angle Spinning.The journal of physical chemistry letters · 2018Article
- Primary Transfer Step in the Light-Driven Ion Pump Bacteriorhodopsin: An Irreversible U-Turn Revealed by Dynamic Nuclear Polarization-Enhanced Magic Angle Spinning NMR.Journal of the American Chemical Society · 2018Article
- Dynamic Nuclear Polarization Signal Enhancement with High-Affinity Biradical Tags.The journal of physical chemistry. B · 2017Article
- Gd(iii) and Mn(ii) complexes for dynamic nuclear polarization: small molecular chelate polarizing agents and applications with site-directed spin labeling of proteins.Physical chemistry chemical physics : PCCP · 2016Article
- Solid-State Dynamic Nuclear Polarization at 9.4 and 18.8 T from 100 K to Room Temperature.Journal of the American Chemical Society · 2015Article
- Mechanisms of dynamic nuclear polarization in insulating solids.Journal of magnetic resonance (San Diego, Calif. : 1997) · 2015Review
- Low-temperature polymorphic phase transition in a crystalline tripeptide L-Ala-L-Pro-Gly·H2O revealed by adiabatic calorimetry.The journal of physical chemistry. B · 2015Article
- Cellular solid-state NMR investigation of a membrane protein using dynamic nuclear polarization.Biochimica et biophysica acta · 2015Article
- Paramagnet induced signal quenching in MAS-DNP experiments in frozen homogeneous solutions.Journal of magnetic resonance (San Diego, Calif. : 1997) · 2014Article
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12 authors.
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Abstract
This contribution addresses four potential misconceptions associated with high-resolution dynamic nuclear polarization/magic angle spinning (DNP/MAS) experiments. First, spectral resolution is not generally compromised at the cryogenic temperatures at which DNP experiments are performed. As we demonstrate at a modest field of 9 T (380 MHz (1)H), 1 ppm linewidths are observed in DNP/MAS spectra of a membrane protein in its native lipid bilayer, and <0.4 ppm linewidths are reported in a crystalline peptide at 85 K. Second, we address the concerns about paramagnetic broadening in DNP/MAS spectra of proteins by demonstrating that the exogenous radical polarizing agents utilized for DNP are distributed in the sample in such a manner as to avoid paramagnetic broadening and thus maintain full spectral resolution. Third, the enhanced polarization is not localized around the polarizing agent, but rather is effectively and uniformly dispersed throughout the sample, even in the case of membrane proteins. Fourth, the distribution of polarization from the electron spins mediated via spin diffusion between (1)H-(1)H strongly dipolar coupled spins is so rapid that shorter magnetization recovery periods between signal averaging transients can be utilized in DNP/MAS experiments than in typical experiments performed at ambient temperature.
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