ArticlePLoS neglected tropical diseases2010
Large scale immune profiling of infected humans and goats reveals differential recognition of Brucella melitensis antigens.
Article in PLoS neglected tropical diseases, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
27 citing papers in PubMed, 43 citations in OpenAlex.
- Development and Preliminary Evaluation of an OMP16-Targeting Trivalent Nanobody-HRP-Based cELISA for Serological Detection of Bovine Brucellosis.Animals : an open access journal from MDPI · 2026Article
- Immuno-profiling ofFrontiers in microbiology · 2023Article
- APRANK: Computational Prioritization of Antigenic Proteins and Peptides From Complete Pathogen Proteomes.Frontiers in immunology · 2021Article
- Proteomics ofMicroorganisms · 2020Article
- Multipathogen Analysis of IgA and IgG Antigen Specificity for Selected Pathogens in Milk Produced by Women From Diverse Geographical Regions: The INSPIRE Study.Frontiers in immunology · 2020Article
- Identification of Cross-Protective Potential Antigens against PathogenicJournal of immunology research · 2018Article
- Identification of Novel Seroreactive Antigens in Johne's Disease Cattle by Using the Mycobacterium tuberculosis Protein Array.Clinical and vaccine immunology : CVI · 2017Article
- Development of ELISAs for diagnosis of acute typhoid fever in Nigerian children.PLoS neglected tropical diseases · 2017Article
- Article
- Review
- A systems biology approach for diagnostic and vaccine antigen discovery in tropical infectious diseases.Current opinion in infectious diseases · 2015Review
- Immune reactivity of sera obtained from brucellosis patients and vaccinated-rabbits to a fusion protein from Brucella melitensis.Iranian journal of basic medical sciences · 2015Article
- A protein-conjugate approach to develop a monoclonal antibody-based antigen detection test for the diagnosis of human brucellosis.PLoS neglected tropical diseases · 2014Article
- Brucella melitensis T cell epitope recognition in humans with brucellosis in Peru.Infection and immunity · 2014Article
- A foodborne outbreak of brucellosis at a police station cafeteria, Lima, Peru.The American journal of tropical medicine and hygiene · 2013Article
- Sustained and differential antibody responses to virulence proteins of Brucella melitensis during acute and chronic infections in human brucellosis.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2013Article
- Article
- Host-Brucella interactions and the Brucella genome as tools for subunit antigen discovery and immunization against brucellosis.Frontiers in cellular and infection microbiology · 2013Review
- Predicting antigenicity of proteins in a bacterial proteome; a protein microarray and naïve Bayes classification approach.Chemistry & biodiversity · 2012Article
- Serial kinetics of the antibody response against the complete Brucella melitensis ORFeome in focal vertebral brucellosis.Journal of clinical microbiology · 2012Article
Corrections and comments
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Authors and funding
18 authors at 8 institutions in 2 countries.
Funding
Abstract
Brucellosis is a widespread zoonotic disease that is also a potential agent of bioterrorism. Current serological assays to diagnose human brucellosis in clinical settings are based on detection of agglutinating anti-LPS antibodies. To better understand the universe of antibody responses that develop after B. melitensis infection, a protein microarray was fabricated containing 1,406 predicted B. melitensis proteins. The array was probed with sera from experimentally infected goats and naturally infected humans from an endemic region in Peru. The assay identified 18 antigens differentially recognized by infected and non-infected goats, and 13 serodiagnostic antigens that differentiate human patients proven to have acute brucellosis from syndromically similar patients. There were 31 cross-reactive antigens in healthy goats and 20 cross-reactive antigens in healthy humans. Only two of the serodiagnostic antigens and eight of the cross-reactive antigens overlap between humans and goats. Based on these results, a nitrocellulose line blot containing the human serodiagnostic antigens was fabricated and applied in a simple assay that validated the accuracy of the protein microarray results in the diagnosis of humans. These data demonstrate that an experimentally infected natural reservoir host produces a fundamentally different immune response than a naturally infected accidental human host.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.