ArticleThe journal of physical chemistry letters2010
Channeling by Proximity: The Catalytic Advantages of Active Site Colocalization Using Brownian Dynamics.
Article in The journal of physical chemistry letters, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 50 papers.
What it found
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Who cites it
50 citing papers in PubMed.
- The Historiography of Galactose and Its Recognition by Galectins.Advances in experimental medicine and biology · 2026Review
- Noncovalently Immobilized Glucose Oxidase/Horseradish Peroxidase Cascade on Polyamide Supports for Eco-Friendly Polyaniline Synthesis.Molecules (Basel, Switzerland) · 2025Article
- Toward Design Principles for Biomolecular Condensates for Metabolic Pathways.Advanced biology · 2025Review
- Designer Glycolysomes: Colocalisation of Glycolytic Enzymes on a Cellulosome-Based Synthetic Protein Scaffold.Microbial biotechnology · 2025Article
- Engineering substrate channeling in a bifunctional terpene synthase.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Non-consecutive enzyme interactions within TCA cycle supramolecular assembly regulate carbon-nitrogen metabolism.Nature communications · 2024Article
- Engineering Substrate Channeling in Assembly-Line Terpene Biosynthesis.bioRxiv : the preprint server for biology · 2024Article
- Enzyme Cascade Electrode Reactions with Nanomaterials and Their Applicability towards Biosensor and Biofuel Cells.Biosensors · 2023Review
- Structural and functional investigations of syn-copalyl diphosphate synthase from Oryza sativa.Communications chemistry · 2023Article
- Enhancing Multistep Reactions: Biomimetic Design of Substrate Channeling Using P22 Virus-Like Particles.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2023Article
- Synthesis of Novel Polymer-Assisted Organic-Inorganic Hybrid Nanoflowers and Their Application in Cascade Biocatalysis.Molecules (Basel, Switzerland) · 2023Article
- Biomanufacturing by In Vitro Biotransformation (ivBT) Using Purified Cascade Multi-enzymes.Advances in biochemical engineering/biotechnology · 2023Article
- Discovery of non-squalene triterpenes.Nature · 2022Article
- Electrostatically cooperative host-in-host of metal cluster ⊂ ionic organic cages in nanopores for enhanced catalysis.Nature communications · 2022Article
- Design of functionalised circular tandem repeat proteins with longer repeat topologies and enhanced subunit contact surfaces.Communications biology · 2021Article
- Metabolic channeling: predictions, deductions, and evidence.Molecular cell · 2021Review
- Substrate Channeling by a Rationally Designed Fusion Protein in a Biocatalytic Cascade.JACS Au · 2021Article
- Systematic mining of fungal chimeric terpene synthases using an efficient precursor-providing yeast chassis.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Structural insight on assembly-line catalysis in terpene biosynthesis.Nature communications · 2021Article
- Cluster Channeling in Cascade Reactions.The journal of physical chemistry. B · 2021Article
Corrections and comments
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Authors and funding
4 authors.
Funding
Abstract
Nature often colocalizes successive steps in a metabolic pathway. Such organization is predicted to increase the effective concentration of pathway intermediates near their recipient active sites and to enhance catalytic efficiency. Here, the pathway of a two-step reaction is modeled using a simple spherical approximation for the enzymes and substrate particles. Brownian dynamics are used to simulate the trajectory of a substrate particle as it diffuses between the active site zones of two different enzyme spheres. The results approximate distances for the most effective reaction pathways, indicating that the most effective reaction pathway is one in which the active sites are closely aligned. However, when the active sites are too close, the ability of the substrate to react with the first enzyme was hindered, suggesting that even the most efficient orientations can be improved for a system that is allowed to rotate or change orientation to optimize the likelihood of reaction at both sites.
Identifiers
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.