ArticleNeoplasia (New York, N.Y.)2010
Three-dimensionally specific inhibition of DNA repair-related genes by activated KRAS in colon crypt model.
Article in Neoplasia (New York, N.Y.), 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 48 citations in OpenAlex.
- A proximity proteomics screen in three-dimensional spheroid cultures identifies novel regulators of lumen formation.Scientific reports · 2021Article
- Common and mutation specific phenotypes of KRAS and BRAF mutations in colorectal cancer cells revealed by integrative -omics analysis.Journal of experimental & clinical cancer research : CR · 2021Article
- Effects ofMicroorganisms · 2021Article
- Predicting the Role of DNA Polymerase β Alone or withJournal of clinical medicine · 2020Article
- A novel compound, ferulic acid-bound resveratrol, induces the tumor suppressor gene p15 and inhibits the three-dimensional proliferation of colorectal cancer cells.Molecular and cellular biochemistry · 2019Article
- Enabling cell recovery from 3D cell culture microfluidic devices for tumour microenvironment biomarker profiling.Scientific reports · 2019Article
- Report of the AAPM TG-256 on the relative biological effectiveness of proton beams in radiation therapy.Medical physics · 2019Article
- A novel resveratrol derivative selectively inhibits the proliferation of colorectal cancer cells with KRAS mutation.Molecular and cellular biochemistry · 2018Article
- The TGF-β pathway is activated by 5-fluorouracil treatment in drug resistant colorectal carcinoma cells.Oncotarget · 2016Article
- ANRIL regulates the proliferation of human colorectal cancer cells in both two- and three-dimensional culture.Molecular and cellular biochemistry · 2016Article
- RAS promotes tumorigenesis through genomic instability induced by imbalanced expression of Aurora-A and BRCA2 in midbody during cytokinesis.International journal of cancer · 2013Article
- Overcoming intratumor heterogeneity of polygenic cancer drug resistance with improved biomarker integration.Neoplasia (New York, N.Y.) · 2012Article
- Inhibition of phosphodiesterase-4 (PDE4) activity triggers luminal apoptosis and AKT dephosphorylation in a 3-D colonic-crypt model.Molecular cancer · 2012Article
- Preclinical study of the DNA repair inhibitor Dbait in combination with chemotherapy in colorectal cancer.Journal of gastroenterology · 2012Article
- Genome-wide profiling of pluripotent cells reveals a unique molecular signature of human embryonic germ cells.PloS one · 2012Article
- The interconnectedness of cancer cell signaling.Neoplasia (New York, N.Y.) · 2011Article
- Oncogenic Kras promotes chemotherapy-induced growth factor shedding via ADAM17.Cancer research · 2011Article
- ZFAT is essential for endothelial cell assembly and the branch point formation of capillary-like structures in an angiogenesis model.Cellular & molecular biology letters · 2010Article
- Dinosaurs and ancient civilizations: reflections on the treatment of cancer.Neoplasia (New York, N.Y.) · 2010Article
- Immune-related zinc finger gene ZFAT is an essential transcriptional regulator for hematopoietic differentiation in blood islands.Proceedings of the National Academy of Sciences of the United States of America · 2010Article
Corrections and comments
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Authors and funding
10 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Growth and differentiation of colonic epithelium are regulated in the three-dimensional (3D) physiological architecture, colonic crypt, and deregulation of 3D interactions is involved in tumorigenesis. Cell-based 3D culture systems provide a suitable approach bridging the gap between two-dimensional (2D) culture and animal models. KRAS mutations are found at high frequencies in human colorectal cancer (CRC); however, KRAS-targeted cancer therapy has not been developed. Here, we have established a 3D cell culture model resembling the colonic crypt by use of HKe3 cells, human CRC HCT116 cells disrupted at activated KRAS. In this 3D colonic crypt model, HKe3 cells showed the features of time course-dependent transit-amplifying and terminal-differentiated stages, which are characteristic of normal colonic crypt. On the basis of the features of HCT116 cells, activated KRAS inhibited normal cell polarity and apoptosis in 3D culture. The expression of DNA repair-related tumor suppressor genes including TP53, BRCA1, BRCA2, and EXO-1 was markedly suppressed by activated KRAS in 3D culture but not in 2D culture. These results together suggest that activated KRAS plays critical roles in the accumulation of genetic alterations through inhibition of DNA repair genes and apoptosis and that this 3D culture model will provide a useful tool for investigating the molecular mechanisms of CRC development.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.