ArticleNature methods2010
A photoconvertible reporter of the ubiquitin-proteasome system in vivo.
Article in Nature methods, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 58 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
58 citing papers in PubMed, 106 citations in OpenAlex.
- Comparative analysis of neuronal proteolytic pathways reveals neuron-specific and sub-compartmental-specific capacities with aging.Cellular and molecular life sciences : CMLS · 2026Article
- Transgenic Dendra2::tau expression allows in vivo monitoring of tau proteostasis in Caenorhabditis elegans.Disease models & mechanisms · 2024Article
- ConsideringCells · 2024Review
- AKIR-1 regulates proteasome subcellular function iniScience · 2023Article
- Visualizing and quantifying molecular and cellular processes in Caenorhabditis elegans using light microscopy.Genetics · 2022Review
- USP10 regulates B cell response to SARS-CoV-2 or HIV-1 nanoparticle vaccines through deubiquitinating AID.Signal transduction and targeted therapy · 2022Article
- Proteasomal subunit depletions differentially affect germline integrity inFrontiers in cell and developmental biology · 2022Article
- Observing and Quantifying Fluorescent Reporters.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Synergistic effects of brain injury and aging: common mechanisms of proteostatic dysfunction.Trends in neurosciences · 2021Review
- Article
- Proteasome activity contributes to pro-survival response upon mild mitochondrial stress in Caenorhabditis elegans.PLoS biology · 2021Article
- The aging proteostasis decline: From nematode to human.Experimental cell research · 2021Review
- Article
- Tissue-specific effects of temperature on proteasome function.Cell stress & chaperones · 2020Article
- Reporter-Based Screens for the Ubiquitin/Proteasome System.Frontiers in chemistry · 2020Review
- The proteostasis network and its decline in ageing.Nature reviews. Molecular cell biology · 2019Review
- Intrinsically aggregation-prone proteins form amyloid-like aggregates and contribute to tissue aging ineLife · 2019Article
- A Practical Review of Proteasome Pharmacology.Pharmacological reviews · 2019Review
- Repeat-associated non-AUG translation in C9orf72-ALS/FTD is driven by neuronal excitation and stress.EMBO molecular medicine · 2019Article
- Visualizing ubiquitination in mammalian cells.EMBO reports · 2019Review
Corrections and comments
- Erratum issued
Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The ubiquitin-proteasome system (UPS) orchestrates many cellular and tissue-specific processes by degrading damaged and key regulatory proteins. To enable investigation of UPS activity in different cell types in a living animal, we developed a photoconvertible fluorescent UPS reporter system for live imaging and quantification of protein degradation in Caenorhabditis elegans. Our reporter consists of the photoconvertible fluorescent protein Dendra2 targeted for proteasomal degradation by fusion to the UbG76V mutant form of ubiquitin. In contrast to previous reporters, this system permits quantification of UPS activity independently of protein synthesis. Our reporter revealed that UPS-mediated protein degradation varies in a cell type-specific and age-dependent manner in C. elegans.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.