ArticleNature structural & molecular biology2010
Nucleotide-induced global conformational changes of flagellar dynein arms revealed by in situ analysis.
Article in Nature structural & molecular biology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 49 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
49 citing papers in PubMed, 106 citations in OpenAlex.
- The Increase in the Frequency and Amplitude of the Beating of Isolated Mouse Tracheal Cilia Reactivated by ATP and cAMP with Elevation in pH.International journal of molecular sciences · 2024Article
- Review
- Design of stimulus-responsive two-state hinge proteins.Science (New York, N.Y.) · 2023Article
- ATP-induced conformational change of axonemal outer dynein arms revealed by cryo-electron tomography.The EMBO journal · 2023Article
- Article
- Regulation of motor activity of ciliary outer-arm dynein by the light chain 1; Implications from the structure of the light chain bound to the microtubule-binding domain of the heavy chain.Biophysics and physicobiology · 2023Article
- Article
- Structure of Motile Cilia.Sub-cellular biochemistry · 2022Article
- Structures of outer-arm dynein array on microtubule doublet reveal a motor coordination mechanism.Nature structural & molecular biology · 2021Article
- Structure and Mechanics of Dynein Motors.Annual review of biophysics · 2021Review
- Structure of a microtubule-bound axonemal dynein.Nature communications · 2021Article
- Force-Generating Mechanism of Axonemal Dynein in Solo and Ensemble.International journal of molecular sciences · 2020Review
- The use of biophysical approaches to understand ciliary beating.Biochemical Society transactions · 2020Review
- Cilia oscillations.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2020Article
- Setting the dynein motor in motion: New insights from electron tomography.The Journal of biological chemistry · 2019Review
- How Does Cilium Length Affect Beating?Biophysical journal · 2019Article
- A microtubule-dynein tethering complex regulates the axonemal inner dynein f (I1).Molecular biology of the cell · 2018Article
- Ciliary Motility: Regulation of Axonemal Dynein Motors.Cold Spring Harbor perspectives in biology · 2017Review
- The tailored sperm cell.Journal of plant research · 2017Review
- Axoneme Structure from Motile Cilia.Cold Spring Harbor perspectives in biology · 2017Review
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Authors and funding
5 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Outer and inner dynein arms generate force for the flagellar/ciliary bending motion. Although nucleotide-induced structural change of dynein heavy chains (the ATP-driven motor) was proven in vitro, our lack of knowledge in situ has precluded an understanding of the bending mechanism. Here we reveal nucleotide-induced global structural changes of the outer and inner dynein arms of Chlamydomonas reinhardtii flagella in situ using electron cryotomography. The ATPase domains of the dynein heavy chains move toward the distal end, and the N-terminal tail bends sharply during product release. This motion could drive the adjacent microtubule to cause a sliding motion. In contrast to in vitro results, in the presence of nucleotides, outer dynein arms coexist as clusters of apo or nucleotide-bound forms in situ. This implies a cooperative switching, which may be related to the mechanism of bending.
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