ArticleAmerican journal of physiology. Lung cellular and molecular physiology2010
Nitric oxide synthase 3 contributes to ventilator-induced lung injury.
Article in American journal of physiology. Lung cellular and molecular physiology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 43 citations in OpenAlex.
- Early effect of tidal volume on lung injury biomarkers in surgical patients with healthy lungs.Anesthesiology · 2014Trial
- Ventilator-Induced Lung Injury: Mechanotransduction and Potential Therapeutic Targets.MedComm · 2026Review
- Characterizing SV40-hTERT Immortalized Human Lung Microvascular Endothelial Cells as Model System for Mechanical Stretch-Induced Lung Injury.International journal of molecular sciences · 2025Article
- NOS3 (rs61722009) gene variants testing in prediction of COVID-19 pneumonia severity.Nitric oxide : biology and chemistry · 2023Article
- Glycyl-L-histidyl-L-lysine-CuFrontiers in molecular biosciences · 2022Article
- Biomechanical Forces and Oxidative Stress: Implications for Pulmonary Vascular Disease.Antioxidants & redox signaling · 2019Review
- Caffeine ameliorates hyperoxia-induced lung injury by protecting GCH1 function in neonatal rat pups.Pediatric research · 2017Article
- Hypoxia treatment reverses neurodegenerative disease in a mouse model of Leigh syndrome.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- Endothelial cell signaling and ventilator-induced lung injury: molecular mechanisms, genomic analyses, and therapeutic targets.American journal of physiology. Lung cellular and molecular physiology · 2017Review
- Parecoxib reduced ventilation induced lung injury in acute respiratory distress syndrome.BMC pharmacology & toxicology · 2017Article
- ROS Signaling in the Pathogenesis of Acute Lung Injury (ALI) and Acute Respiratory Distress Syndrome (ARDS).Advances in experimental medicine and biology · 2017Article
- Article
- Nox2-dependent glutathionylation of endothelial NOS leads to uncoupled superoxide production and endothelial barrier dysfunction in acute lung injury.American journal of physiology. Lung cellular and molecular physiology · 2014Article
- Nitric oxide synthase promotes distension-induced tracheal venular leukocyte adherence.PloS one · 2014Article
- Hemoglobin infusion does not alter murine pulmonary vascular tone.Nitric oxide : biology and chemistry · 2013Article
- Inhaled nitric oxide attenuates the adverse effects of transfusing stored syngeneic erythrocytes in mice with endothelial dysfunction after hemorrhagic shock.Anesthesiology · 2012Article
- Ventilation with "clinically relevant" high tidal volumes does not promote stretch-induced injury in the lungs of healthy mice.Critical care medicine · 2012Article
- Pulmonary microRNA profiling in a mouse model of ventilator-induced lung injury.American journal of physiology. Lung cellular and molecular physiology · 2012Article
- Activation of calpains mediates early lung neutrophilic inflammation in ventilator-induced lung injury.American journal of physiology. Lung cellular and molecular physiology · 2012Article
- Protective and Detrimental Effects of Sodium Sulfide and Hydrogen Sulfide in Murine Ventilator-induced Lung Injury.Anesthesiology · 2011Article
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
Nitric oxide synthase (NOS) depletion or inhibition reduces ventilator-induced lung injury (VILI), but the responsible mechanisms remain incompletely defined. The aim of this study was to elucidate the role of endothelial NOS, NOS3, in the pathogenesis of VILI in an in vivo mouse model. Wild-type and NOS3-deficient mice were ventilated with high-tidal volume (HV(T); 40 ml/kg) for 4 h, with and without adding NO to the inhaled gas. Additional wild-type mice were pretreated with tetrahydrobiopterin and ascorbic acid, agents that can prevent NOS-generated superoxide production. Arterial blood gas tensions, histology, and lung mechanics were evaluated after 4 h of HV(T) ventilation. The concentration of protein, IgM, cytokines, malondialdehyde, and 8-isoprostane were measured in bronchoalveolar lavage fluid (BALF). Myeloperoxidase activity, total and oxidized glutathione levels, and NOS-derived superoxide production were measured in lung tissue homogenates. HV(T) ventilation induced VILI in wild-type mice, as reflected by decreased lung compliance, increased concentrations of protein and cytokines in BALF, and oxidative stress. All indices of VILI were ameliorated in NOS3-deficient mice. Augmenting pulmonary NO levels by breathing NO during mechanical ventilation did not increase lung injury in NOS3-deficient mice. HV(T) ventilation increased NOS-inhibitable superoxide production in lung extracts from wild-type mice but not in those from NOS3-deficient mice. Administration of tetrahydrobiopterin and ascorbic acid ameliorated VILI in wild-type mice. Our results indicate that NOS3 contributes to ventilator-induced lung injury via increased production of superoxide.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.