ArticleAmerican journal of physiology. Lung cellular and molecular physiology2010
cGMP increases antioxidant function and attenuates oxidant cell death in mouse lung microvascular endothelial cells by a protein kinase G-dependent mechanism.
Article in American journal of physiology. Lung cellular and molecular physiology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 39 citations in OpenAlex.
- Redox Regulation of Microvascular Physiology and Pathophysiology: Insights into Therapeutic Strategies and Limitations.Antioxidants & redox signaling · 2025Review
- Pharmacological activation of BK channels protects against LPS-induced pneumonia.Scientific reports · 2025Article
- GUCY2C signaling limits dopaminergic neuron vulnerability to toxic insults.NPJ Parkinson's disease · 2024Article
- BMSC-Derived Exosomes Carrying lncRNA-ZFAS1 Alleviate Pulmonary Ischemia/Reperfusion Injury by UPF1-Mediated mRNA Decay of FOXD1.Molecular neurobiology · 2023Article
- Intra-amniotic sildenafil treatment improves lung blood flow and pulmonary hypertension in congenital diaphragmatic hernia rats.Frontiers in bioengineering and biotechnology · 2023Article
- Endothelial colony-forming cells reduced the lung injury induced by cardiopulmonary bypass in rats.Stem cell research & therapy · 2020Article
- rBMSC/Cav-1Stem cells international · 2019Article
- A biplot correlation range for group-wise metabolite selection in mass spectrometry.BioData mining · 2019Article
- Transepithelial Fluid and Salt Re-Absorption Regulated by cGK2 Signals.International journal of molecular sciences · 2018Review
- Stimulators and activators of soluble guanylate cyclase for urogenital disorders.Nature reviews. Urology · 2018Review
- Hyper-activation of pp60Free radical biology & medicine · 2017Article
- Hydrogen peroxide-induced calcium influx in lung microvascular endothelial cells involves TRPV4.American journal of physiology. Lung cellular and molecular physiology · 2015Article
- The tyrosine kinase inhibitor imatinib prevents lung injury and death after intravenous LPS in mice.Physiological reports · 2015Article
- Protein kinase G increases antioxidant function in lung microvascular endothelial cells by inhibiting the c-Abl tyrosine kinase.American journal of physiology. Cell physiology · 2014Article
- Excessive mechanical stress increases HMGB1 expression in human lung microvascular endothelial cells via STAT3.Microvascular research · 2014Article
- CD36 and Fyn kinase mediate malaria-induced lung endothelial barrier dysfunction in mice infected with Plasmodium berghei.PloS one · 2013Article
- Article
- The pulmonary endothelial glycocalyx regulates neutrophil adhesion and lung injury during experimental sepsis.Nature medicine · 2012Article
- Natriuretic peptide receptor guanylyl cyclase-A protects podocytes from aldosterone-induced glomerular injury.Journal of the American Society of Nephrology : JASN · 2012Article
- Protein kinase G-I deficiency induces pulmonary hypertension through Rho A/Rho kinase activation.The American journal of pathology · 2012Article
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
Increasing evidence suggests that endothelial cytotoxicity from reactive oxygen species (ROS) contributes to the pathogenesis of acute lung injury. Treatments designed to increase intracellular cGMP attenuate ROS-mediated apoptosis and necrosis in several cell types, but the mechanisms are not understood, and the effect of cGMP on pulmonary endothelial cell death remains controversial. In the current study, increasing intracellular cGMP by either 8pCPT-cGMP (50 microM) or atrial natriuretic peptide (10 nM) significantly attenuated cell death in H(2)O(2)-challenged mouse lung microvascular (MLMVEC) monolayers. 8pCPT-cGMP also decreased perfusate LDH release in isolated mouse lungs exposed to H(2)O(2) or ischemia-reperfusion. The protective effect of increasing cGMP in MLMVECs was accompanied by enhanced endothelial H(2)O(2) scavenging (measured by H(2)O(2) electrode) and decreased intracellular ROS concentration (measured by 2',7'-dichlorofluorescin fluorescence) as well as decreased phosphorylation of p38 MAPK and Akt. The cGMP-mediated cytoprotection and increased H(2)O(2) scavenging required >2 h of 8pCPT-cGMP incubation in wild-type MLMVEC and were absent in MLMVEC from protein kinase G (PKG(I))-/- mice suggesting a PKG(I)-mediated effect on gene regulation. Catalase and glutathione peroxidase 1 (Gpx-1) protein were increased by cGMP in wild-type but not PKG(I)-/- MLMVEC monolayers. Both the cGMP-mediated increases in antioxidant proteins and H(2)O(2) scavenging were prevented by inhibition of translation with cycloheximide. 8pCPT-cGMP had minimal effects on catalase and Gpx-1 mRNA. We conclude that cGMP, through PKG(I), attenuated H(2)O(2)-induced cytotoxicity in MLMVEC by increasing catalase and Gpx-1 expression through an unknown posttranscriptional effect.
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