ArticleApplied and environmental microbiology2010
Comparison of different DNA-based methods for molecular typing of Histoplasma capsulatum.
Article in Applied and environmental microbiology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers, 1 of them a synthesis that pooled it.
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Who cites it
20 citing papers in PubMed, 1 synthesis or guideline pooled it, 49 citations in OpenAlex.
- The use of genetic markers in the molecular epidemiology of histoplasmosis: a systematic review.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2016Pooled it
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- Sporotrichosis Caused by Non-Wild TypeFrontiers in cellular and infection microbiology · 2022Article
- Environmental Isolation ofFrontiers in cellular and infection microbiology · 2022Article
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- The global epidemiology of emergingStudies in mycology · 2020Article
- Primary Central Nervous System Infection by Histoplasma in an Immunocompetent Adult.Mycopathologia · 2020Article
- Postpartum histoplasmosis in an HIV-negative woman: a case report and phylogenetic characterization by internal transcribed spacer region analysis.Revista da Sociedade Brasileira de Medicina Tropical · 2020Article
- How Environmental Fungi Cause a Range of Clinical Outcomes in Susceptible Hosts.Journal of molecular biology · 2019Review
- Mixed infection by Histoplasma capsulatum isolates with different mating types in Brazilian AIDS-patients.Revista do Instituto de Medicina Tropical de Sao Paulo · 2019Article
- Multiplex polymerase chain reaction as an improved method for screening Histoplasma capsulatum mating types.Memorias do Instituto Oswaldo Cruz · 2018Article
- Development and Evaluation of a Molecular Diagnostic Method for Rapid Detection of Histoplasma capsulatum var. farciminosum, the Causative Agent of Epizootic Lymphangitis, in Equine Clinical Samples.Journal of clinical microbiology · 2016Article
- Worldwide Phylogenetic Distributions and Population Dynamics of the Genus Histoplasma.PLoS neglected tropical diseases · 2016Article
- Evaluation of neonatal Toll-like receptors 3 (c.1377C/T) and 9 (G2848A) gene polymorphisms in HBV intrauterine transmission susceptibility.Epidemiology and infection · 2015Article
- Histoplasmosis in HIV-Infected Patients: A Review of New Developments and Remaining Gaps.Current tropical medicine reports · 2014Review
- Frequency and genetic diversity of the MAT1 locus of Histoplasma capsulatum isolates in Mexico and Brazil.Eukaryotic cell · 2013Article
- Disseminated histoplasmosis in a domestic cat imported from the USA to Austria.Medical mycology case reports · 2013Article
- Epidemiology of Invasive Fungal Infections in Latin America.Current fungal infection reports · 2012Article
- Fatal Histoplasma capsulatum mitral endocarditis in a French patient treated for rheumatoid arthritis.Mycopathologia · 2012Article
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 3 countries.
Funding
Abstract
Histoplasma capsulatum is very prevalent in the environment and is one of the most common causes of mycoses in humans and diverse animals in Brazil. Multiple typing methods have been developed to study H. capsulatum epidemiology; however, there is limited information concerning comparisons of results obtained with different methods using the same set of isolates. To explore the diversity of H. capsulatum in Brazil and to determine correlations between the results of three different molecular typing techniques, we examined 51 environmental, animal, and human isolates by M13 PCR fingerprinting, PCR-restriction fragment length polymorphism (RFLP) analysis of the internal transcribed region 1 (ITS1)-5.8S-ITS2 region of the rDNA locus, and DNA sequencing and phylogenetic analysis of parts of four protein-encoding genes, the Arf (ADP ribosylation factor), H-anti (H antigen precursor), Ole (delta-9 fatty acid desaturase), and Tub1 (alpha-tubulin) genes. Each method identified three major genetic clusters, and there was a high level of concordance between the results of the typing techniques. The M13 PCR fingerprinting and PCR-RFLP analyses produced very similar results and separated the H. capsulatum isolates included in this study into three major groups. An additional approach used was comparison of our Brazilian ITS1-5.8S-ITS2 sequences with the sequences deposited previously in NCBI data banks. Our analyses suggest that H. capsulatum can be divided into different molecular types that are dispersed around the world. Our results indicate that the three methods used in this study are reliable and reproducible and that they have similar sensitivities. However, M13 PCR fingerprinting has some advantages over the other two methods as it is faster, cheaper, and more user friendly, which especially increases its utility for molecular typing of Histoplasma in situations where laboratory facilities are relatively limited.
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