ArticleApplied and environmental microbiology2010
Disruption of the putative cell surface polysaccharide biosynthesis gene SO3177 in Shewanella oneidensis MR-1 enhances adhesion to electrodes and current generation in microbial fuel cells.
Article in Applied and environmental microbiology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.
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Who cites it
38 citing papers in PubMed, 156 citations in OpenAlex.
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- A non-methanogenic archaeon within the order Methanocellales.Nature communications · 2024Article
- Statistical optimization of waste molasses-based exopolysaccharides and self-sustainable bioelectricity production for dual chamber microbial fuel cell by Bacillus piscis.Microbial cell factories · 2023Article
- Enhanced depolluting capabilities of microbial bioelectrochemical systems by synthetic biology.Synthetic and systems biotechnology · 2023Article
- Moving towards the enhancement of extracellular electron transfer in electrogens.World journal of microbiology & biotechnology · 2023Review
- Over-expressing NadA quinolinate synthase in Escherichia coli enhances the bioelectrochemistry in microbial fuel cells.Biology open · 2023Article
- Systematic Full-Cycle Engineering Microbial Biofilms to Boost Electricity Production inResearch (Washington, D.C.) · 2023Article
- Type I-F CRISPR-PAIR platform for multi-mode regulation to boost extracellular electron transfer iniScience · 2022Article
- Boosting microfluidic microbial fuel cells performance via investigating electron transfer mechanisms, metal-based electrodes, and magnetic field effect.Scientific reports · 2022Article
- Microbiomics for enhancing electron transfer in an electrochemical system.Frontiers in microbiology · 2022Review
- Extracellular riboflavin induces anaerobic biofilm formation in Shewanella oneidensis.Biotechnology for biofuels · 2021Article
- Identification of a Diguanylate Cyclase That Facilitates Biofilm Formation on Electrodes by Shewanella oneidensis MR-1.Applied and environmental microbiology · 2021Article
- Towards Application of Electro-Fermentation for the Production of Value-Added Chemicals From Biomass Feedstocks.Frontiers in chemistry · 2021Review
- Single cell electron collectors for highly efficient wiring-up electronic abiotic/biotic interfaces.Nature communications · 2020Article
- Elucidating the Role of Virulence Traits in the Survival of PathogenicFrontiers in bioengineering and biotechnology · 2020Article
- Electricigens in the anode of microbial fuel cells: pure cultures versus mixed communities.Microbial cell factories · 2019Review
- Roles of d-Lactate Dehydrogenases in the Anaerobic Growth ofApplied and environmental microbiology · 2019Article
- A Synthetic Plasmid Toolkit forFrontiers in microbiology · 2019Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A microbial fuel cell (MFC) was inoculated with a random transposon insertion mutant library of Shewanella oneidensis MR-1 and operated with lactate as the sole fuel to select for mutants that preferentially grew in it. Agar plate cultivation of the resultant MFC enrichment culture detected an increased number of colonies exhibiting rough morphology. One such isolate, strain 4A, generated 50% more current in an MFC than wild-type MR-1. Determination of the transposon insertion site in strain 4A followed by deletion and complementation experiments revealed that the SO3177 gene, encoding a putative formyltransferase and situated in a cell surface polysaccharide biosynthesis gene cluster, was responsible for the increased current. Transmission electron microscopy showed that a layered structure at the cell surface, stainable with ruthenium red, was impaired in the SO3177 mutant (DeltaSO3177), confirming that SO3177 is involved in the biosynthesis of cell surface polysaccharides. Compared to the wild type, DeltaSO3177 cells preferentially attached to graphite felt anodes in MFCs, while physicochemical analyses revealed that the cell surface of DeltaSO3177 was more hydrophobic. These results demonstrate that cell surface polysaccharides affect not only the cell adhesion to graphite anodes but also the current generation in MFCs.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.