ArticleApplied and environmental microbiology2010
Shotgun proteomics of Aspergillus niger microsomes upon D-xylose induction.
Article in Applied and environmental microbiology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 40 citations in OpenAlex.
- Exposure ofFrontiers in microbiology · 2019Article
- Extracellular vesicles carry cellulases in the industrial fungusBiotechnology for biofuels · 2019Article
- The Peroxygenase Activity of theFrontiers in microbiology · 2018Article
- Identification of a Novel L-rhamnose Uptake Transporter in the Filamentous Fungus Aspergillus niger.PLoS genetics · 2016Article
- The amyR-deletion strain of Aspergillus niger CICC2462 is a suitable host strain to express secreted protein with a low background.Microbial cell factories · 2016Article
- A two-dimensional protein map of Pleurotus ostreatus microsomes-proteome dynamics.Folia microbiologica · 2016Article
- Evidence of cAMP involvement in cellobiohydrolase expression and secretion by Trichoderma reesei in presence of the inducer sophorose.BMC microbiology · 2015Article
- A Caleosin-Like Protein with Peroxygenase Activity Mediates Aspergillus flavus Development, Aflatoxin Accumulation, and Seed Infection.Applied and environmental microbiology · 2015Article
- Methodologies and perspectives of proteomics applied to filamentous fungi: from sample preparation to secretome analysis.International journal of molecular sciences · 2015Review
- Aspergillus niger membrane-associated proteome analysis for the identification of glucose transporters.Biotechnology for biofuels · 2015Article
- Genome-scale analysis of the high-efficient protein secretion system of Aspergillus oryzae.BMC systems biology · 2014Article
- Secretory pathway of cellulase: a mini-review.Biotechnology for biofuels · 2013Article
- Proteomics of industrial fungi: trends and insights for biotechnology.Applied microbiology and biotechnology · 2011Review
- Proteomic analysis of the secretory response of Aspergillus niger to D-maltose and D-xylose.PloS one · 2011Article
Corrections and comments
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4 authors at 1 institution in 1 country.
Funding
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Abstract
Protein secretion plays an eminent role in cell maintenance and adaptation to the extracellular environment of microorganisms. Although protein secretion is an extremely efficient process in filamentous fungi, the mechanisms underlying protein secretion have remained largely uncharacterized in these organisms. In this study, we analyzed the effects of the d-xylose induction of cellulase and hemicellulase enzyme secretion on the protein composition of secretory organelles in Aspergillus niger. We aimed to systematically identify the components involved in the secretion of these enzymes via mass spectrometry of enriched subcellular microsomal fractions. Under each condition, fractions enriched for secretory organelles were processed for tandem mass spectrometry, resulting in the identification of peptides that originate from 1,081 proteins, 254 of which-many of them hypothetical proteins-were predicted to play direct roles in the secretory pathway. d-Xylose induction led to an increase in specific small GTPases known to be associated with polarized growth, exocytosis, and endocytosis. Moreover, the endoplasmic-reticulum-associated degradation (ERAD) components Cdc48 and all 14 of the 20S proteasomal subunits were recruited to the secretory organelles. In conclusion, induction of extracellular enzymes results in specific changes in the secretory subproteome of A. niger, and the most prominent change found in this study was the recruitment of the 20S proteasomal subunits to the secretory organelles.
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