ArticleJournal of bacteriology2010
Mutations in the Bacillus subtilis beta clamp that separate its roles in DNA replication from mismatch repair.
Article in Journal of bacteriology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 20 citations in OpenAlex.
- The B. subtilis translesion polymerase Pol Y1 is not strongly recruited to sites of replication upon different types of DNA damage.PLoS genetics · 2026Article
- "ThebioRxiv : the preprint server for biology · 2026Article
- Binding of the regulatory domain of MutL to the sliding β-clamp is species specific.Nucleic acids research · 2019Article
- MutS2 Promotes Homologous Recombination in Bacillus subtilis.Journal of bacteriology · 2017Article
- Addressing the Requirements of High-Sensitivity Single-Molecule Imaging of Low-Copy-Number Proteins in Bacteria.Chemphyschem : a European journal of chemical physics and physical chemistry · 2016Article
- Single-molecule motions and interactions in live cells reveal target search dynamics in mismatch repair.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- RecO and RecR are necessary for RecA loading in response to DNA damage and replication fork stress.Journal of bacteriology · 2014Article
- RecD2 helicase limits replication fork stress in Bacillus subtilis.Journal of bacteriology · 2014Article
- Cost of rNTP/dNTP pool imbalance at the replication fork.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- DnaN clamp zones provide a platform for spatiotemporal coupling of mismatch detection to DNA replication.Molecular microbiology · 2013Article
- DNA Mismatch Repair.EcoSal Plus · 2012Article
- Residues in the N-terminal domain of MutL required for mismatch repair in Bacillus subtilis.Journal of bacteriology · 2012Article
- DNA repair and genome maintenance in Bacillus subtilis.Microbiology and molecular biology reviews : MMBR · 2012Review
- Mismatch repair causes the dynamic release of an essential DNA polymerase from the replication fork.Molecular microbiology · 2011Article
Corrections and comments
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The beta clamp is an essential replication sliding clamp required for processive DNA synthesis. The beta clamp is also critical for several additional aspects of DNA metabolism, including DNA mismatch repair (MMR). The dnaN5 allele of Bacillus subtilis encodes a mutant form of beta clamp containing the G73R substitution. Cells with the dnaN5 allele are temperature sensitive for growth due to a defect in DNA replication at 49 degrees C, and they show an increase in mutation frequency caused by a partial defect in MMR at permissive temperatures. We selected for intragenic suppressors of dnaN5 that rescued viability at 49 degrees C to determine if the DNA replication defect could be separated from the MMR defect. We isolated three intragenic suppressors of dnaN5 that restored growth at the nonpermissive temperature while maintaining an increase in mutation frequency. All three dnaN alleles encoded the G73R substitution along with one of three novel missense mutations. The missense mutations isolated were S22P, S181G, and E346K. Of these, S181G and E346K are located near the hydrophobic cleft of the beta clamp, a common site occupied by proteins that bind the beta clamp. Using several methods, we show that the increase in mutation frequency resulting from each dnaN allele is linked to a defect in MMR. Moreover, we found that S181G and E346K allowed growth at elevated temperatures and did not have an appreciable effect on mutation frequency when separated from G73R. Thus, we found that specific residue changes in the B. subtilis beta clamp separate the role of the beta clamp in DNA replication from its role in MMR.
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