ArticleNucleic acids research2010
Rapid decay of unstable Leishmania mRNAs bearing a conserved retroposon signature 3'-UTR motif is initiated by a site-specific endonucleolytic cleavage without prior deadenylation.
Article in Nucleic acids research, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
19 citing papers in PubMed, 33 citations in OpenAlex.
- Article
- Evolutionary divergent clusters of transcribed extinct truncated retroposons drive low mRNA expression and developmental regulation in the protozoan Leishmania.BMC biology · 2024Article
- In Vivo Tethering System to Isolate RNA-Binding Proteins Regulating mRNA Decay in Leishmania.Methods in molecular biology (Clifton, N.J.) · 2020Article
- Gene and Chromosomal Copy Number Variations as an Adaptive Mechanism Towards a Parasitic Lifestyle in Trypanosomatids.Current genomics · 2018Review
- The Pumilio-domain protein PUF6 contributes to SIDER2 retroposon-mediated mRNA decay inRNA (New York, N.Y.) · 2017Article
- Article
- Disclosing 3' UTR cis-elements and putative partners involved in gene expression regulation in Leishmania spp.PloS one · 2017Article
- Plasticity of theF1000Research · 2016Review
- Drug resistance analysis by next generation sequencing in Leishmania.International journal for parasitology. Drugs and drug resistance · 2015Review
- Species-specific antimonial sensitivity in Leishmania is driven by post-transcriptional regulation of AQP1.PLoS neglected tropical diseases · 2015Article
- A short RNA stem-loop is necessary and sufficient for repression of gene expression during early logarithmic phase in trypanosomes.Nucleic acids research · 2014Article
- The wide expansion of hepatitis delta virus-like ribozymes throughout trypanosomatid genomes is linked to the spreading of L1Tc/ingi clade mobile elements.BMC genomics · 2014Article
- The roles of 3'-exoribonucleases and the exosome in trypanosome mRNA degradation.RNA (New York, N.Y.) · 2013Article
- The structure and repertoire of small interfering RNAs in Leishmania (Viannia) braziliensis reveal diversification in the trypanosomatid RNAi pathway.Molecular microbiology · 2013Article
- Pr77 and L1TcRz: A dual system within the 5'-end of L1Tc retrotransposon, internal promoter and HDV-like ribozyme.Mobile genetic elements · 2012Article
- Article
- TSIDER1, a short and non-autonomous Salivarian trypanosome-specific retroposon related to the ingi6 subclade.Molecular and biochemical parasitology · 2011Article
- Novel endoribonucleases as central players in various pathways of eukaryotic RNA metabolism.RNA (New York, N.Y.) · 2010Review
- PUF Proteins as Critical RNA-Binding Proteins in TriTryp Parasites: A Review Article.Iranian journal of parasitologyReview
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
Abstract
We have previously shown that the Leishmania genome possess two widespread families of extinct retroposons termed Short Interspersed DEgenerated Retroposons (SIDER1/2) that play a role in post-transcriptional regulation. Moreover, we have demonstrated that SIDER2 retroposons promote mRNA degradation. Here we provide new insights into the mechanism by which unstable Leishmania mRNAs harboring a SIDER2 retroposon in their 3'-untranslated region are degraded. We show that, unlike most eukaryotic transcripts, SIDER2-bearing mRNAs do not undergo poly(A) tail shortening prior to rapid turnover, but instead, they are targeted for degradation by a site-specific endonucleolytic cleavage. The main cleavage site was mapped in two randomly selected SIDER2-containing mRNAs in vivo between an AU dinucleotide at the 5'-end of the second 79-nt signature (signature II), which represents the most conserved sequence amongst SIDER2 retroposons. Deletion of signature II abolished endonucleolytic cleavage and deadenylation-independent decay and increased mRNA stability. Interestingly, we show that overexpression of SIDER2 anti-sense RNA can increase sense transcript abundance and stability, and that complementarity to the cleavage region is required for protecting SIDER2-containing transcripts from degradation. These results establish a new paradigm for how unstable mRNAs are degraded in Leishmania and could serve as the basis for a better understanding of mRNA decay pathways in general.
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Registered trials
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