ArticleThe Journal of biological chemistry2010
Temperature-sensitive post-translational regulation of plant omega-3 fatty-acid desaturases is mediated by the endoplasmic reticulum-associated degradation pathway.
Article in The Journal of biological chemistry, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
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37 citing papers in PubMed, 88 citations in OpenAlex.
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- Mutual antagonism between auxin and diacylglycerol acyltransferase 2 regulates high-temperature-induced hypocotyl elongation.The New phytologist · 2026Article
- Fatty Acid Metabolism in Shading-Induced Tolerance of Star Ruby Grapefruit to Postharvest Chilling Injury.Plants (Basel, Switzerland) · 2025Article
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- Quality assessment of flax advanced breeding lines varying in seed coat color and their potential use in the food and industrial applications.BMC plant biology · 2024Article
- Temperature and elevated COFrontiers in plant science · 2024Article
- Heat stress during seed development leads to impaired physiological function and plasticity in seed oil accumulation inFrontiers in plant science · 2023Article
- Hot topic: Thermosensing in plants.Plant, cell & environment · 2021Review
- Temperature Acclimation of the PicoalgaFrontiers in plant science · 2021Article
- Remodeling of theVaccines · 2020Article
- Design of high-oleic tobacco (Nicotiana tabacum L.) seed oil by CRISPR-Cas9-mediated knockout of NtFAD2-2.BMC plant biology · 2020Article
- Isolation and functional analyses ofPeerJ · 2020Article
- Development of aFrontiers in plant science · 2020Article
- Interspecific Variation in the Unsaturation Level of Seed Oils Were Associated With the Expression Pattern Shifts of Duplicated Desaturase Genes and the Potential Role of Other Regulatory Genes.Frontiers in plant science · 2020Article
- Plant Unsaturated Fatty Acids: Biosynthesis and Regulation.Frontiers in plant science · 2020Review
- Plasticity of fruit and oil traits in olive among different environments.Scientific reports · 2019Article
- Cellular Organization and Regulation of Plant Glycerolipid Metabolism.Plant & cell physiology · 2019Review
- Effect of high night temperature on storage lipids and transcriptome changes in developing seeds of oilseed rape.Journal of experimental botany · 2018Article
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Authors and funding
9 authors at 5 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Changes in ambient temperature represent a major physiological challenge to membranes of poikilothermic organisms. In plants, the endoplasmic reticulum (ER)-localized omega-3 fatty-acid desaturases (Fad3) increase the production of polyunsaturated fatty acids at cooler temperatures, but the FAD3 genes themselves are typically not up-regulated during this adaptive response. Here, we expressed two closely related plant FAD3 genes in yeast cells and found that their enzymes produced significantly different amounts of omega-3 fatty acids and that these differences correlated to differences in rates of protein turnover. Domain-swapping and mutagenesis experiments revealed that each protein contained a degradation signal in its N terminus and that the charge density of a PEST-like sequence within this region was largely responsible for the differences in rates of protein turnover. The half-life of each Fad3 protein was increased at cooler temperatures, and protein degradation required specific components of the ER-associated degradation pathway including the Cdc48 adaptor proteins Doa1, Shp1, and Ufd2. Expression of the Fad3 proteins in tobacco cells incubated with the proteasomal inhibitor MG132 further confirmed that they were degraded via the proteasomal pathway in plants. Collectively, these findings indicate that Fad3 protein abundance is regulated by a combination of cis-acting degradation signals and the ubiquitin-proteasome pathway and that modulation of Fad3 protein amounts in response to temperature may represent one mechanism of homeoviscous adaptation in plants.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.