ArticleThe international journal of biochemistry & cell biology2010
Activation of Erk by sonic hedgehog independent of canonical hedgehog signalling.
Article in The international journal of biochemistry & cell biology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
36 citing papers in PubMed, 61 citations in OpenAlex.
- Sonic Hedgehog Signaling Pathway: A Role in Pain Processing.Neurochemical research · 2023Review
- Patched regulates lipid homeostasis by controlling cellular cholesterol levels.Nature communications · 2021Article
- Hedgehog Signaling and Truncated GLI1 in Cancer.Cells · 2020Review
- Circadian BMAL1 regulates mandibular condyle development by hedgehog pathway.Cell proliferation · 2020Article
- Sonic hedgehog enhances calcium oscillations in hippocampal astrocytes.The Journal of biological chemistry · 2019Article
- Hedgehog Signaling: An Achilles' Heel in Cancer.Translational oncology · 2019Review
- Review
- Review
- The protein-specific activities of the transmembrane modules of Ptch1 and Ptch2 are determined by their adjacent protein domains.The Journal of biological chemistry · 2018Article
- Sonic Hedgehog Signaling Pathway Mediates Proliferation and Migration of Fibroblast-Like Synoviocytes in Rheumatoid ArthritisFrontiers in immunology · 2018Article
- New paradigms for the Hedgehog signaling network in mammary gland development and breast Cancer.Biochimica et biophysica acta. Reviews on cancer · 2017Review
- Epithelial and non-epithelialDevelopment (Cambridge, England) · 2017Article
- Comparative Analysis of Mutational Profile of Sonic hedgehog Gene in Gallbladder Cancer.Digestive diseases and sciences · 2017Article
- What Is Breast in the Bone?International journal of molecular sciences · 2016Review
- Activities of the Cytoplasmic Domains of Patched-1 Modulate but Are Not Essential for the Regulation of Canonical Hedgehog Signaling.The Journal of biological chemistry · 2016Article
- Hedgehog Signaling in Pancreatic Fibrosis and Cancer.Medicine · 2016Review
- Ptch2 loss drives myeloproliferation and myeloproliferative neoplasm progression.The Journal of experimental medicine · 2016Article
- Review
- Hedgehog Acyltransferase as a target in estrogen receptor positive, HER2 amplified, and tamoxifen resistant breast cancer cells.Molecular cancer · 2015Article
- The Role of ARF6 in Biliary Atresia.PloS one · 2015Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
Abstract
Hedgehog (Hh) signalling is mediated through the Patched-1 (Ptch1) receptor. Hh-binding to Ptch1 blocks the inhibitory effects of Ptch1 on the activity of the transmembrane protein, Smoothened (Smo), resulting induction of target genes by the Gli-family of transcription factors. We demonstrate here that Hh-binding to Ptch1 stimulates activation of Erk1/2. This activation is insensitive to the small molecule Smo antagonists and occurs in a cell line that does not express Smo. Specifically, the C-terminus of Ptch1 harbours motifs encoding Class I and II SH3-binding sites. SH3-domain binding activity was verified using GST-c-src(SH3), -Grb2(SH3) and -p85beta(SH3) fusion-proteins. Ectopically expressed Grb2 or p85beta could also be co-immunoprecipitated with the Ptch1 C-terminus. Addition of Shh to serum-starved human mammary epithelial cells and Shh Light II fibroblasts stimulated phosphorylation of Erk1/2. Erk1/2 activation was observed in cells where Smo activity had been inhibited using cyclopamine and in the breast epithelial cell line, MCF10A, that does not express Smo. These data reveal novel binding activities for the C-terminal region of Ptch1 and define a signalling pathway stimulated by the Hh-ligands operating independently of pathways requiring Smo.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.