ArticleBiochimica et biophysica acta2010
Altered plasmodial surface anion channel activity and in vitro resistance to permeating antimalarial compounds.
Article in Biochimica et biophysica acta, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 18 citations in OpenAlex.
- Development of Semisynthetic Blasticidin S Analogs with Potent and Fast-Killing Antimalarial Activity.ACS infectious diseases · 2026Article
- Novel Ion Channel Genes in Malaria Parasites.Genes · 2024Review
- Critical interdependencies between Plasmodium nutrient flux and drugs.Trends in parasitology · 2023Review
- Transporter-Mediated Solutes Uptake as Drug Target inFrontiers in pharmacology · 2022Review
- Identification of Antimalarial Compounds That Require CLAG3 for Their Uptake byAntimicrobial agents and chemotherapy · 2019Article
- A CLAG3 mutation in an amphipathic transmembrane domain alters malaria parasite nutrient channels and confers leupeptin resistance.Infection and immunity · 2015Article
- Potent Plasmodium falciparum gametocytocidal activity of diaminonaphthoquinones, lead antimalarial chemotypes identified in an antimalarial compound screen.Antimicrobial agents and chemotherapy · 2015Article
- How do antimalarial drugs reach their intracellular targets?Frontiers in pharmacology · 2015Review
- The conserved clag multigene family of malaria parasites: essential roles in host-pathogen interaction.Drug resistance updates : reviews and commentaries in antimicrobial and anticancer chemotherapy · 2015Review
- Why do malaria parasites increase host erythrocyte permeability?Trends in parasitology · 2014Review
- High guanidinium permeability reveals dehydration-dependent ion selectivity in the plasmodial surface anion channel.BioMed research international · 2014Article
- Epigenetic switches in clag3 genes mediate blasticidin S resistance in malaria parasites.Cellular microbiology · 2013Article
- An epigenetic antimalarial resistance mechanism involving parasite genes linked to nutrient uptake.The Journal of biological chemistry · 2013Article
- Ion and nutrient uptake by malaria parasite-infected erythrocytes.Cellular microbiology · 2012Review
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
Abstract
Erythrocytes infected with malaria parasites have increased permeability to various solutes. These changes may be mediated by an unusual small conductance ion channel known as the plasmodial surface anion channel (PSAC). While channel activity benefits the parasite by permitting nutrient acquisition, it can also be detrimental because water-soluble antimalarials may more readily access their parasite targets via this channel. Recently, two such toxins, blasticidin S and leupeptin, were used to select mutant parasites with altered PSAC activities, suggesting acquired resistance via reduced channel-mediated toxin uptake. Surprisingly, although these toxins have similar structures and charge, we now show that reduced permeability of one does not protect the intracellular parasite from the other. Leupeptin accumulation in the blasticidin S-resistant mutant was relatively preserved, consistent with retained in vitro susceptibility to leupeptin. Subsequent in vitro selection with both toxins generated a double mutant parasite having additional changes in PSAC, implicating an antimalarial resistance mechanism for water-soluble drugs requiring channel-mediated uptake at the erythrocyte membrane. Characterization of these mutants revealed a single conserved channel on each mutant, albeit with distinct gating properties. These findings are consistent with a shared channel that mediates uptake of ions, nutrients and toxins. This channel's gating and selectivity properties can be modified in response to in vitro selective pressure.
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