ArticleArchives of biochemistry and biophysics2010
Purification and characterization of Put1p from Saccharomyces cerevisiae.
Article in Archives of biochemistry and biophysics, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 39 citations in OpenAlex.
- Mechanisms of formic acid stress response in evolved yeast for microbial-electrocatalytic systems.Biodesign research · 2026Article
- Please, carefully, pass the P5C.Journal of experimental botany · 2024Article
- Evidence for Proline Catabolic Enzymes in the Metabolism of Thiazolidine Carboxylates.Biochemistry · 2021Article
- Appropriate Activity Assays Are Crucial for the Specific Determination of Proline Dehydrogenase and Pyrroline-5-Carboxylate Reductase Activities.Frontiers in plant science · 2020Article
- Proline Metabolism in Cell Regulation and Cancer Biology: Recent Advances and Hypotheses.Antioxidants & redox signaling · 2019Review
- Structural Biology of Proline Catabolic Enzymes.Antioxidants & redox signaling · 2019Review
- Role of Proline in Pathogen and Host Interactions.Antioxidants & redox signaling · 2019Review
- The Proline Cycle As a Potential Cancer Therapy Target.Biochemistry · 2018Article
- Article
- Structure, function, and mechanism of proline utilization A (PutA).Archives of biochemistry and biophysics · 2017Review
- Evidence for Pipecolate Oxidase in Mediating Protection Against Hydrogen Peroxide Stress.Journal of cellular biochemistry · 2017Article
- Co-regulation of mitochondrial respiration by proline dehydrogenase/oxidase and succinate.Amino acids · 2016Article
- Hyperprolinemia in Type 2 Glutaric Aciduria and MADD-Like Profiles.JIMD reports · 2016Article
- Proline dehydrogenase 2 (PRODH2) is a hydroxyproline dehydrogenase (HYPDH) and molecular target for treating primary hyperoxaluria.The Biochemical journal · 2015Article
- Role of proline and pyrroline-5-carboxylate metabolism in plant defense against invading pathogens.Frontiers in plant science · 2015Review
- Structural studies of yeast Δ(1)-pyrroline-5-carboxylate dehydrogenase (ALDH4A1): active site flexibility and oligomeric state.Biochemistry · 2014Article
- L-Proline uptake in Saccharomyces cerevisiae mitochondria can contribute to bioenergetics during nutrient stress as alternative mitochondrial fuel.World journal of microbiology & biotechnology · 2014Article
- Sources of superoxide/H2O2 during mitochondrial proline oxidation.Redox biology · 2014Article
- Article
- Proline mechanisms of stress survival.Antioxidants & redox signaling · 2013Review
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
In Saccharomyces cerevisiae, the PUT1 and PUT2 genes are required for the conversion of proline to glutamate. The PUT1 gene encodes Put1p, a proline dehydrogenase (PRODH) enzyme localized in the mitochondrion. Put1p was expressed and purified from Escherichia coli and shown to have a UV-visible absorption spectrum that is typical of a bound flavin cofactor. A K(m) value of 36 mM proline and a k(cat)=27 s(-1) were determined for Put1p using an artificial electron acceptor. Put1p also exhibited high activity using ubiquinone-1 (CoQ(1)) as an electron acceptor with a k(cat)=9.6 s(-1) and a K(m) of 33 microM for CoQ(1). In addition, knockout strains of the electron transfer flavoprotein (ETF) homolog in S. cerevisiae were able to grow on proline as the sole nitrogen source demonstrating that ETF is not required for proline utilization in yeast.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.