ReviewApplied microbiology and biotechnology2010
Inteins, valuable genetic elements in molecular biology and biotechnology.
Review in Applied microbiology and biotechnology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 42 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
42 citing papers in PubMed, 118 citations in OpenAlex.
- Regulation of N-terminal cleavage and splicing in mini-Ter DnaE-3 intein by E23G and R50Q mutations.Biotechnology letters · 2026Article
- Approaches to Study Proteins Encoded by Essential Genes.Proteins · 2026Review
- Article
- Intein-Mediated Protein Engineering for Cell-Based Biosensors.Biosensors · 2022Review
- SufB intein splicing in Mycobacterium tuberculosis is influenced by two remote conserved N-extein histidines.Bioscience reports · 2022Article
- Use Intein Cleavable Polyhydroxyalkanoate Synthase Fusions to Improve Protein Solubility.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Comparative Genomics of Three Novel Jumbo Bacteriophages Infecting Staphylococcus aureus.Journal of virology · 2021Article
- Discovering the N-Terminal Methylome by Repurposing of Proteomic Datasets.Journal of proteome research · 2021Article
- A systematic approach to inserting split inteins for Boolean logic gate engineering and basal activity reduction.Nature communications · 2021Article
- The Effects of Self-cleavage Intein-ELK16 Tag in the Transcript Steric Hindrance of IFN.Indian journal of clinical biochemistry : IJCB · 2021Article
- Inteins in Science: Evolution to Application.Microorganisms · 2020Review
- Article
- Large nanodiscs going viral.Current opinion in structural biology · 2020Review
- Methods to Study the Structure and Catalytic Activity of cis-Splicing Inteins.Methods in molecular biology (Clifton, N.J.) · 2020Article
- Expanding the Diversity ofViruses · 2019Article
- Effect of Amino Acid Substitutions on Biological Activity of Antimicrobial Peptide: Design, Recombinant Production, and Biological Activity.Iranian journal of pharmaceutical research : IJPR · 2019Article
- Covalently circularized nanodiscs; challenges and applications.Current opinion in structural biology · 2018Review
- The dynamic intein landscape of eukaryotes.Mobile DNA · 2018Article
- Microbial lipolytic fusion enzymes: current state and future perspectives.World journal of microbiology & biotechnology · 2017Review
- Purification of Antibacterial CHAPProbiotics and antimicrobial proteins · 2016Article
Corrections and comments
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Authors and funding
2 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Inteins are internal protein elements that self-excise from their host protein and catalyze ligation of the flanking sequences (exteins) with a peptide bond. They are found in organisms in all three domains of life, and in viral proteins. Intein excision is a posttranslational process that does not require auxiliary enzymes or cofactors. This self-excision process is called protein splicing, by analogy to the splicing of RNA introns from pre-mRNA. Protein splicing involves only four intramolecular reactions, and a small number of key catalytic residues in the intein and exteins. Protein-splicing can also occur in trans. In this case, the intein is separated into N- and C-terminal domains, which are synthesized as separate components, each joined to an extein. The intein domains reassemble and link the joined exteins into a single functional protein. Understanding the cis- and trans-protein splicing mechanisms led to the development of intein-mediated protein-engineering applications, such as protein purification, ligation, cyclization, and selenoprotein production. This review summarizes the catalytic activities and structures of inteins, and focuses on the advantages of some recent intein applications in molecular biology and biotechnology.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.