ArticleEMBO reports2010
Molecular origin of plasma membrane citrate transporter in human prostate epithelial cells.
Article in EMBO reports, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
25 citing papers in PubMed, 42 citations in OpenAlex.
- Boosting the Activity of Melanoma-Targeting CAR-T Cells in the Presence of Citrate by the Application of Gluconate.Pharmaceutics · 2026Article
- The Influence of Extracellular Citrate in Physiological Concentration on the Proliferation of Malignant Melanoma.Journal of cellular and molecular medicine · 2026Article
- Mechanisms of seminal plasma synthesis and actions on sperm function for advancing reproductive technologies.The Journal of reproduction and development · 2026Review
- The Blood-prostate Barrier: An Obstacle to Drug Delivery into the Prostate.Current drug delivery · 2025Review
- Membrane transporter progressive ankylosis protein homologue (Frontiers in aging · 2025Article
- Extreme mitochondrial reduction in a novel group of free-living metamonads.Nature communications · 2024Article
- Emerging Perspectives in Zinc Transporter Research in Prostate Cancer: An Updated Review.Nutrients · 2024Review
- Expression of the plasma membrane citrate carrier (pmCiC) in human cancerous tissues-correlation with tumour aggressiveness.Frontiers in cell and developmental biology · 2024Article
- Hypoxia induced lactate acidosis modulates tumor microenvironment and lipid reprogramming to sustain the cancer cell survival.Frontiers in oncology · 2023Review
- Carbon sources and pathways for citrate secreted by human prostate cancer cells determined by NMR tracing and metabolic modeling.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Metabolic Alterations in Cellular Senescence: The Role of Citrate in Ageing and Age-Related Disease.International journal of molecular sciences · 2022Review
- Extracellular citrate and metabolic adaptations of cancer cells.Cancer metastasis reviews · 2021Review
- Cancer-associated cells release citrate to support tumour metastatic progression.Life science alliance · 2021Article
- The Mitochondrial Citrate Carrier SLC25A1/CIC and the Fundamental Role of Citrate in Cancer, Inflammation and Beyond.Biomolecules · 2021Review
- An Overview of Cell-Based Assay Platforms for the Solute Carrier Family of Transporters.Frontiers in pharmacology · 2021Review
- Article
- Review
- The membrane protein ANKH is crucial for bone mechanical performance by mediating cellular export of citrate and ATP.PLoS genetics · 2020Article
- Tumour metabolism and its unique properties in prostate adenocarcinoma.Nature reviews. Urology · 2020Review
- Extracellular Citrate Fuels Cancer Cell Metabolism and Growth.Frontiers in cell and developmental biology · 2020Review
Corrections and comments
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Authors and funding
13 authors at 6 institutions in 5 countries.
Funding
Abstract
The prostate is a highly specialized mammalian organ that produces and releases large amounts of citrate. However, the citrate release mechanism is not known. Here, we present the results of molecular cloning of a citrate transporter from human normal prostate epithelial PNT2-C2 cells shown previously to express such a mechanism. By using rapid amplification of cDNA ends PCR, we determined that the prostatic carrier is an isoform of the mitochondrial transporter SLC25A1 with a different first exon. We confirmed the functionality of the clone by expressing it in human embryonic kidney cells and performing radiotracer experiments and whole-cell patch-clamp recordings. By using short interfering RNAs targeting different parts of the sequence, we confirmed that the cloned protein is the main prostatic transporter responsible for citrate release. We also produced a specific antibody and localized the cloned transporter protein to the plasma membrane of the cells. By using the same antibody, we have shown that the cloned transporter is expressed in non-malignant human tissues.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.