ArticleUltrasound in medicine & biology2010
Saving cells from ultrasound-induced apoptosis: quantification of cell death and uptake following sonication and effects of targeted calcium chelation.
Article in Ultrasound in medicine & biology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed.
- The molecular impact of sonoporation: A transcriptomic analysis of gene regulation profile.Ultrasonics sonochemistry · 2024Article
- Article
- Application of nanosonosensitizer materials in cancer sono-dynamic therapy.RSC advances · 2022Review
- An Overview of Cell Membrane Perforation and Resealing Mechanisms for Localized Drug Delivery.Pharmaceutics · 2022Review
- Vaccine and vaccination as a part of human life: In view of COVID-19.Biotechnology journal · 2022Review
- Sonoporation generates downstream cellular impact after membrane resealing.Scientific reports · 2021Article
- Enhanced Radiosensitization for Cancer Treatment with Gold Nanoparticles through Sonoporation.International journal of molecular sciences · 2020Article
- Damage- and pathogen-associated molecular patterns play differential roles in late mortality after critical illness.JCI insight · 2019Article
- Intracellular Delivery by Membrane Disruption: Mechanisms, Strategies, and Concepts.Chemical reviews · 2018Review
- Prolonging pulse duration in ultrasound-mediated gene delivery lowers acoustic pressure threshold for efficient gene transfer to cells and small animals.Journal of controlled release : official journal of the Controlled Release Society · 2018Article
- Nanoparticle-assisted ultrasound: A special focus on sonodynamic therapy against cancer.Chemical engineering journal (Lausanne, Switzerland : 1996) · 2018Article
- Effects of combined delivery of extremely low frequency electromagnetic field and magnetic Fe3O4 nanoparticles on hepatic cell lines.American journal of translational research · 2016Article
- In vitro methods to study bubble-cell interactions: Fundamentals and therapeutic applications.Biomicrofluidics · 2016Review
- Treatment of hepatic carcinoma by low-frequency ultrasound and microbubbles: A case report.Oncology letters · 2015Article
- Synergistic delivery of gold nanorods using multifunctional microbubbles for enhanced plasmonic photothermal therapy.Scientific reports · 2014Article
- Mechanisms of microbubble-facilitated sonoporation for drug and gene delivery.Therapeutic delivery · 2014Review
- The induction of the apoptosis of cancer cell by sonodynamic therapy: a review.Chinese journal of cancer research = Chung-kuo yen cheng yen chiu · 2012Article
- Different effects of sonoporation on cell morphology and viability.Bosnian journal of basic medical sciences · 2012Article
- Can ultrasound enable efficient intracellular uptake of molecules? A retrospective literature review and analysis.Ultrasound in medicine & biology · 2012Review
Corrections and comments
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Authors and funding
4 authors.
Funding
Abstract
Applications of ultrasound for noninvasive drug and gene delivery have been limited by associated cell death as a result of sonication. In this study, we sought to quantify the distribution of cellular bioeffects caused by low-frequency ultrasound (24 kHz) and test the hypothesis that Ca(2+) chelation after sonication can shift this distribution by saving cells from death by apoptosis. Using flow cytometry, we quantitatively categorized sonicated cells among four populations: (i) cells that appear largely unaffected, (ii) cells reversibly permeabilized, (iii) cells rendered nonviable during sonication and (iv) cells that appear to be viable shortly after sonication, but later undergo apoptosis and die. By monitoring cells for 6 h after ultrasound exposure, we found that up to 15% of intact cells fell into this final category. Those apoptotic cells initially had the highest levels of uptake of a marker compound, calcein; also had highly elevated levels of intracellular Ca(2+); and contained an estimated plasma membrane wound radius of 100-300 nm. Finally, we showed that chelation of intracellular Ca(2+) after sonication reduced apoptosis by up to 44%, thereby providing a strategy to save cells. We conclude that cells can be saved from ultrasound-induced death by appropriate selection of ultrasound conditions and Ca(2+) chelation after sonication.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.