ArticleProtein expression and purification2010
Expression, purification, and characterization of recombinant human transferrin from rice (Oryza sativa L.).
Article in Protein expression and purification, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed.
- Production of recombinant human transferrin using transgenic rice cell culture.Plant biotechnology (Tokyo, Japan) · 2026Article
- Animal-derived components in cultivated meat research and their alternatives.NPJ science of food · 2025Review
- The challenges of using fish cells for cultivated seafood production.Food science and biotechnology · 2025Review
- A review and outlook on expression of animal proteins in plants.Frontiers in plant science · 2024Review
- Seed- and leaf-based expression of FGF21-transferrin fusion proteins for oral delivery and treatment of non-alcoholic steatohepatitis.Frontiers in plant science · 2022Article
- Characterization and Oral Delivery of Proinsulin-Transferrin Fusion Protein Expressed Using ExpressTec.International journal of molecular sciences · 2018Article
- Clinical grade manufacturing of genetically modified, CAR-expressing NK-92 cells for the treatment of ErbB2-positive malignancies.Cancer immunology, immunotherapy : CII · 2018Article
- Application of recombinant human leukemia inhibitory factor (LIF) produced in rice (Oryza sativa L.) for maintenance of mouse embryonic stem cells.Journal of biotechnology · 2014Article
- Immunotoxicity assessment of rice-derived recombinant human serum albumin using human peripheral blood mononuclear cells.PloS one · 2014Article
- N-glycosylation and N-glycan moieties of CTB expressed in rice seeds.Molecular biotechnology · 2013Article
- Characterization of transferrin receptor-mediated endocytosis and cellular iron delivery of recombinant human serum transferrin from rice (Oryza sativa L.).BMC biotechnology · 2012Article
- Biochemical and structural characterization of recombinant human serum transferrin from rice (Oryza sativa L.).Journal of inorganic biochemistry · 2012Article
- The long history of iron in the Universe and in health and disease.Biochimica et biophysica acta · 2012Review
- Transferrin-mediated cellular iron delivery.Current topics in membranes · 2012Review
- High-level production of animal-free recombinant transferrin from Saccharomyces cerevisiae.Microbial cell factories · 2010Article
- Analysis of carbohydrates and glycoconjugates by matrix-assisted laser desorption/ionization mass spectrometry: an update for 2009-2010.Mass spectrometry reviewsReview
- Article
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Authors and funding
7 authors.
Funding
Abstract
Transferrin is an essential ingredient used in cell culture media due to its crucial role in regulating cellular iron uptake, transport, and utilization. It is also a promising drug carrier used to increase a drug's therapeutic index via the unique transferrin receptor-mediated endocytosis pathway. Due to the high risk of contamination with blood-borne pathogens from the use of human or animal plasma-derived transferrin, recombinant transferrin is preferred for use as a replacement for native transferrin. We expressed recombinant human transferrin in rice (Oryza sativa L.) at a high level of 1% seed dry weight (10 g/kg). The recombinant human transferrin was able to be extracted with saline buffers and then purified by a one step anion exchange chromatographic process to greater than 95% purity. The rice-derived recombinant human transferrin was shown to be not only structurally similar to the native human transferrin, but also functionally the same as native transferrin in terms of reversible iron binding and promoting cell growth and productivity. These results indicate that rice-derived recombinant human transferrin should be a safe and low cost alternative to human or animal plasma-derived transferrin for use in cell culture-based biopharmaceutical production of protein therapeutics and vaccines.
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Registered trials
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