ArticleJournal of neuroscience methods2010
Collection of nanoliter microdialysate fractions in plugs for off-line in vivo chemical monitoring with up to 2 s temporal resolution.
Article in Journal of neuroscience methods, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
30 citing papers in PubMed, 61 citations in OpenAlex.
- Sampling Probes Microfabricated from Parylene‑C for In Vivo Neurochemical Monitoring.ACS measurement science au · 2025Article
- 4D-Printed Redox-Responsive Needle-Flow Reactors Enabling Online Quantitative Profiling of Living Rat Brain Extracellular Lactate and Glucose.Analytical chemistry · 2025Article
- Microdialysis coupled with droplet microfluidics and mass spectrometry for determination of neurotransmittersThe Analyst · 2024Article
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- Droplet-assisted electrospray phase separation using an integrated silicon microfluidic platform.Lab on a chip · 2021Article
- Dexamethasone-Enhanced Microdialysis and Penetration Injury.Frontiers in bioengineering and biotechnology · 2020Review
- Review
- Use and Future Prospects of in Vivo Microdialysis for Epilepsy Studies.ACS chemical neuroscience · 2019Review
- In Vivo Chemical Monitoring at High Spatiotemporal Resolution Using Microfabricated Sampling Probes and Droplet-Based Microfluidics Coupled to Mass Spectrometry.Analytical chemistry · 2018Article
- Measurement of Basal Neurotransmitter Levels Using Convolution-Based Nonfaradaic Current Removal.Analytical chemistry · 2018Article
- Microfabricated Probes for Studying Brain Chemistry: A Review.Chemphyschem : a European journal of chemical physics and physical chemistry · 2018Review
- Hitchhiker's Guide to Voltammetry: Acute and Chronic Electrodes for in Vivo Fast-Scan Cyclic Voltammetry.ACS chemical neuroscience · 2017Review
- Microfabrication and in Vivo Performance of a Microdialysis Probe with Embedded Membrane.Analytical chemistry · 2016Article
- Mass spectrometric analysis of spatio-temporal dynamics of crustacean neuropeptides.Biochimica et biophysica acta · 2015Review
- Development of an on-animal separation-based sensor for monitoring drug metabolism in freely roaming sheep.The Analyst · 2015Article
- A review of flux considerations for in vivo neurochemical measurements.The Analyst · 2015Review
- A review of microdialysis coupled to microchip electrophoresis for monitoring biological events.Journal of chromatography. A · 2015Review
- Electrochemical Analysis of Neurotransmitters.Annual review of analytical chemistry (Palo Alto, Calif.) · 2015Review
- Microelectronics-based biosensors dedicated to the detection of neurotransmitters: a review.Sensors (Basel, Switzerland) · 2014Review
- Capillary liquid chromatography fraction collection and postcolumn reaction using segmented flow microfluidics.Journal of separation science · 2013Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
An off-line in vivo neurochemical monitoring approach was developed based on collecting nanoliter microdialysate fractions as an array of "plugs" segmented by immiscible oil in a piece of Teflon tubing. The dialysis probe was integrated with the plug generator in a polydimethlysiloxane microfluidic device that could be mounted on the subject. The microfluidic device also allowed derivatization reagents to be added to the plugs for fluorescence detection of analytes. Using the device, 2 nL fractions corresponding to 1-20 ms sampling times depending upon dialysis flow rate, were collected. Because axial dispersion was prevented between them, each plug acted as a discrete sample collection vial and temporal resolution was not lost by mixing or diffusion during transport. In vitro tests of the system revealed that the temporal resolution of the system was as good as 2 s and was limited by mass transport effects within the dialysis probe. After collection of dialysate fractions, they were pumped into a glass microfluidic chip that automatically analyzed the plugs by capillary electrophoresis with laser-induced fluorescence at 50 s intervals. By using a relatively low flow rate during transfer to the chip, the temporal resolution of the samples could be preserved despite the relatively slow analysis time. The system was used to detect rapid dynamics in neuroactive amino acids evoked by microinjecting the glutamate uptake inhibitor l-trans-pyrrolidine-2,4-dicarboxylic acid (PDC) or K(+) into the striatum of anesthetized rats. The resulted showed increases in neurotransmitter efflux that reached a peak in 20 s for PDC and 13 s for K(+).
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.