ArticleArchives of biochemistry and biophysics2010
Transport and equilibrium uptake of a peptide inhibitor of PACE4 into articular cartilage is dominated by electrostatic interactions.
Article in Archives of biochemistry and biophysics, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
22 citing papers in PubMed.
- Spatial charge-hydrophobicity configuration modulates cationic peptide transport in cartilage.Biophysical journal · 2025Article
- Identification of Genes Associated with Liver Metastasis in Pancreatic Cancer Reveals PCSK6 as a Crucial Mediator.Cancers · 2022Article
- The Role of Proprotein Convertases in Upper Airway Remodeling.Molecules and cells · 2022Review
- Article
- Multi-Trait Genome-Wide Association Study of Atherosclerosis Detects Novel Pleiotropic Loci.Frontiers in genetics · 2021Article
- Overcoming negatively charged tissue barriers: Drug delivery using cationic peptides and proteins.Nano today · 2020Article
- Green fluorescent proteins engineered for cartilage-targeted drug delivery: Insights for transport into highly charged avascular tissues.Biomaterials · 2018Article
- Pulsed gradient stimulated echo (PGStE) NMR shows spatial dependence of fluid diffusion in human stage IV osteoarthritic cartilage.Magnetic resonance in medicine · 2018Article
- Molecular transport in articular cartilage - what have we learned from the past 50 years?Nature reviews. Rheumatology · 2018Review
- Synthesis and Preclinical Characterization of a Cationic Iodinated Imaging Contrast Agent (CA4+) and Its Use for Quantitative Computed Tomography of Ex Vivo Human Hip Cartilage.Journal of medicinal chemistry · 2017Article
- Review
- Cartilage-targeting drug delivery: can electrostatic interactions help?Nature reviews. Rheumatology · 2017Review
- Bridge-enhanced ACL repair: A review of the science and the pathway through FDA investigational device approval.Annals of biomedical engineering · 2015Review
- Tantalum oxide nanoparticles for the imaging of articular cartilage using X-ray computed tomography: visualization of ex vivo/in vivo murine tibia and ex vivo human index finger cartilage.Angewandte Chemie (International ed. in English) · 2014Article
- Sustained delivery of bioactive TGF-β1 from self-assembling peptide hydrogels induces chondrogenesis of encapsulated bone marrow stromal cells.Journal of biomedical materials research. Part A · 2014Article
- Avidin as a model for charge driven transport into cartilage and drug delivery for treating early stage post-traumatic osteoarthritis.Biomaterials · 2014Article
- Transport and binding of tumor necrosis factor-α in articular cartilage depend on its quaternary structure.Archives of biochemistry and biophysics · 2013Article
- Spatial configuration and composition of charge modulates transport into a mucin hydrogel barrier.Biophysical journal · 2013Article
- Transport of anti-IL-6 antigen binding fragments into cartilage and the effects of injury.Archives of biochemistry and biophysics · 2013Article
- Article
Corrections and comments
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Authors and funding
6 authors.
Funding
Abstract
The availability of therapeutic molecules to targets within cartilage depends on transport through the avascular matrix. We studied equilibrium partitioning and non-equilibrium transport into cartilage of Pf-pep, a 760 Da positively charged peptide inhibitor of the proprotein convertase PACE4. Competitive binding measurements revealed negligible binding of Pf-pep to sites within cartilage. Uptake of Pf-pep depended on glycosaminoglycan charge density, and was consistent with predictions of Donnan equilibrium given the known charge of Pf-pep. In separate transport experiments, the diffusivity of Pf-pep in cartilage was measured to be approximately 1 x 10(-6) cm(2)/s, close to other similarly-sized non-binding solutes. These results suggest that small positively charged therapeutics will have a higher concentration within cartilage than in the surrounding synovial fluid, a desired property for local delivery; however, such therapeutics may rapidly diffuse out of cartilage unless there is additional specific binding to intra-tissue substrates that can maintain enhanced intra-tissue concentration for local delivery.
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