ArticleMolecular plant pathology2010
PopW of Ralstonia solanacearum, a new two-domain harpin targeting the plant cell wall.
Article in Molecular plant pathology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 50 citations in OpenAlex.
- Selected cell wall-associated components in plant defense responses against microbial pathogens.Frontiers in plant science · 2026Review
- Review
- HrpW Modulates Paracidovorax citrulli Virulence and Plant Immunity via ClRAR1 Interaction in Watermelon.Molecular plant pathology · 2025Article
- Unraveling the genomic complexity of secretion systems in the most virulent Xanthomonas arboricola pathovars.PloS one · 2025Article
- The Carbonic Anhydrase βCA1 Functions in PopW-Mediated Plant Defense Responses in Tomato.International journal of molecular sciences · 2023Article
- Characteristics, Roles and Applications of Proteinaceous Elicitors from Pathogens in Plant Immunity.Life (Basel, Switzerland) · 2023Review
- The application of exogenous PopW increases the tolerance ofPhysiology and molecular biology of plants : an international journal of functional plant biology · 2020Article
- The stability of the coiled-coil structure near to N-terminus influence the heat resistance of harpin proteins from Xanthomonas.BMC microbiology · 2020Article
- The signal peptide-like segment of hpaXm is required for its association to the cell wall in transgenic tobacco plants.PloS one · 2017Article
- Plant Resistance Inducers against Pathogens in Solanaceae Species-From Molecular Mechanisms to Field Application.International journal of molecular sciences · 2016Review
- Overexpression of a harpin-encoding gene popW from Ralstonia solanacearum primed antioxidant defenses with enhanced drought tolerance in tobacco plants.Plant cell reports · 2016Article
- Comparative Secretome Analysis of Ralstonia solanacearum Type 3 Secretion-Associated Mutants Reveals a Fine Control of Effector Delivery, Essential for Bacterial Pathogenicity.Molecular & cellular proteomics : MCP · 2016Article
- Key steps in type III secretion system (T3SS) towards translocon assembly with potential sensor at plant plasma membrane.Molecular plant pathology · 2015Review
- Ectopically expressed sweet pepper ferredoxin PFLP enhances disease resistance to Pectobacterium carotovorum subsp. carotovorum affected by harpin and protease-mediated hypersensitive response in Arabidopsis.Molecular plant pathology · 2014Article
- Commonalities and differences of T3SSs in rhizobia and plant pathogenic bacteria.Frontiers in plant science · 2014Review
- Repertoire, unified nomenclature and evolution of the Type III effector gene set in the Ralstonia solanacearum species complex.BMC genomics · 2013Article
- A highly-conserved single-stranded DNA-binding protein in Xanthomonas functions as a harpin-like protein to trigger plant immunity.PloS one · 2013Article
- Protein export according to schedule: architecture, assembly, and regulation of type III secretion systems from plant- and animal-pathogenic bacteria.Microbiology and molecular biology reviews : MMBR · 2012Review
- A novel antimicrobial protein for plant protection consisting of a Xanthomonas oryzae harpin and active domains of cecropin A and melittin.Microbial biotechnology · 2011Article
- Hpa2 required by HrpF to translocate Xanthomonas oryzae transcriptional activator-like effectors into rice for pathogenicity.Applied and environmental microbiology · 2011Article
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Authors and funding
7 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Harpins are extracellular glycine-rich proteins eliciting a hypersensitive response (HR). In this study, we identified a new harpin, PopW, from Ralstonia solanacearum strain ZJ3721. This 380-amino-acid protein is acidic, rich in glycine and serine, and lacks cysteine. When infiltrated into the leaves of tobacco (non-host), PopW induced a rapid tissue collapse via a heat-stable but protease-sensitive HR-eliciting activity. PopW has an N-terminal harpin domain (residues 1-159) and a C-terminal pectate lyase (PL) domain (residues 160-366); its HR-eliciting activity depends on its N-terminal domain. Analyses of subcellular localization and plasmolysis demonstrated that PopW targeted the onion cell wall. This was further confirmed by its ability to specifically bind to calcium pectate, a major component of the plant cell wall. However, PopW had no detectable PL activity. Western blotting revealed that PopW was secreted by the type III secretion system in an hrpB-dependent manner. Gene sequencing indicated that popW is conserved among 20 diverse strains of R. solanacearum. A popW-deficient mutant retained the ability of wild-type strain ZJ3721 to elicit HR in tobacco and to cause wilt disease in tomato (a host). We conclude that PopW is a new cell wall-associated, hrpB-dependent, two-domain harpin that is conserved across the R. solanacearum species complex.
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