ArticleMolecular plant pathology2010
High-throughput sequencing of Hop stunt viroid-derived small RNAs from cucumber leaves and phloem.
Article in Molecular plant pathology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed, 74 citations in OpenAlex.
- Small RNAs derived from avocado sunblotch viroid and their association with bleaching symptoms: implications for pathogenesis in avocado sunblotch disease.Archives of virology · 2025Article
- Viroids: Non-Coding Circular RNAs Able to Autonomously Replicate and Infect Higher Plants.Biology · 2023Review
- A circular RNA vector for targeted plant gene silencing based on an asymptomatic viroid.The Plant journal : for cell and molecular biology · 2022Article
- Reprogramming of RNA silencing triggered by cucumber mosaic virus infection in Arabidopsis.Genome biology · 2021Article
- Hop stunt viroid: A polyphagous pathogenic RNA that has shed light on viroid-host interactions.Molecular plant pathology · 2021Article
- Effects of Host-Adaptive Mutations on Hop Stunt Viroid Pathogenicity and Small RNA Biogenesis.International journal of molecular sciences · 2020Article
- Impact of Nucleic Acid Sequencing on Viroid Biology.International journal of molecular sciences · 2020Review
- Citrus exocortis viroid causes ribosomal stress in tomato plants.Nucleic acids research · 2019Article
- Article
- Next-Generation Sequencing and CRISPR/Cas13 Editing in Viroid Research and Molecular Diagnostics.Viruses · 2019Review
- Small RNA-based antimicrobial immunity.Nature reviews. Immunology · 2019Review
- Article
- Transcriptome sequencing reveals novel Citrus bark cracking viroid (CBCVd) variants from citrus and their molecular characterization.PloS one · 2018Article
- In silico analysis of the grapefruit sRNAome, transcriptome and gene regulation in response to CTV-CDVd co-infection.Virology journal · 2017Article
- Identification and characterization of microRNAs in Humulus lupulus using high-throughput sequencing and their response to Citrus bark cracking viroid (CBCVd) infection.BMC genomics · 2016Article
- Changes in the DNA methylation pattern of the host male gametophyte of viroid-infected cucumber plants.Journal of experimental botany · 2016Article
- Next-Generation Sequencing and Genome Editing in Plant Virology.Frontiers in microbiology · 2016Review
- Comparative analysis among the small RNA populations of source, sink and conductive tissues in two different plant-virus pathosystems.BMC genomics · 2015Article
- Discovery of replicating circular RNAs by RNA-seq and computational algorithms.PLoS pathogens · 2014Article
- What has been happening with viroids?Virus genes · 2014Review
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Small RNA (sRNA)-guided processes, referred to as RNA silencing, regulate endogenous and exogenous gene expression. In plants and some animals, these processes are noncell autonomous and can operate beyond the site of initiation. Viroids, the smallest self-replicating plant pathogens known, are inducers, targets and evaders of this regulatory mechanism and, consequently, the presence of viroid-derived sRNAs (vd-sRNAs) is usually associated with viroid infection. However, the pathways involved in the biogenesis of vd-sRNAs are largely unknown. Here, we analyse, by high-throughput pyrosequencing, the profiling of the Hop stunt viroid (HSVd) vd-sRNAs recovered from the leaves and phloem of infected cucumber (Cucumis sativus) plants. HSVd vd-sRNAs are mostly 21 and 22 nucleotides in length and derived equally from plus and minus HSVd RNA strands. The widespread distribution of vd-sRNAs across the genome reveals that the totality of the HSVd RNA genome contributes to the formation of vd-sRNAs. Our sequence data suggest that viroid-derived double-stranded RNA functions as one of the main precursors of vd-sRNAs. Remarkably, phloem vd-sRNAs accumulated preferentially as 22-nucleotide species with a consensus sequence over-represented. This bias in size and sequence in the HSVd vd-sRNA population recovered from phloem exudate suggests the existence of a selective trafficking of vd-sRNAs to the phloem tissue of infected cucumber plants.
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Registered trials
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