ArticleMolecular plant pathology2010
The 3'-terminal sequence of Bamboo mosaic virus minus-strand RNA interacts with RNA-dependent RNA polymerase and initiates plus-strand RNA synthesis.
Article in Molecular plant pathology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
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Who cites it
6 citing papers in PubMed, 8 citations in OpenAlex.
- RNA Viral Vectors for Accelerating Plant Synthetic Biology.Frontiers in plant science · 2021Article
- Autophagy is involved in assisting the replication of Bamboo mosaic virus in Nicotiana benthamiana.Journal of experimental botany · 2019Article
- Function and Structural Organization of the Replication Protein ofFrontiers in microbiology · 2017Review
- The Functional Roles of theFrontiers in microbiology · 2017Review
- Promotion of Bamboo Mosaic Virus Accumulation in Nicotiana benthamiana by 5'→3' Exonuclease NbXRN4.Frontiers in microbiology · 2015Article
- The conserved 5' apical hairpin stem loops of bamboo mosaic virus and its satellite RNA contribute to replication competence.Nucleic acids research · 2012Article
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
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Abstract
A 3'-terminal, 77-nucleotide sequence of Bamboo mosaic virus (BaMV) minus-strand RNA (Ba-77), comprising a 5' stem-loop, a spacer and a 3'-CUUUU sequence, can be used to initiate plus-strand RNA synthesis in vitro. To understand the mechanism of plus-strand RNA synthesis, mutations were introduced in the 5' untranslated region of BaMV RNA, resulting in changes at the 3' end of minus-strand RNA. The results showed that at least three uridylate residues in 3'-CUUUU are required and the changes at the penultimate U are deleterious to viral accumulation in Nicotiana benthamiana protoplasts. Results from UV-crosslinking and in vitro RNA-dependent RNA polymerase competition assays suggested that the replicase preferentially interacts with the stem structure of Ba-77. Finally, CMV/83 + UUUUC, a heterologus RNA, which possesses about 80 nucleotides containing the 3'-CUUUU pentamer terminus, and which folds into a secondary structure similar to that of Ba-77, could be used as template for RNA production by the BaMV replicase complex in vitro.
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Registered trials
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