ArticleJournal of the American Chemical Society2010
Mechanisms of quenching of Alexa fluorophores by natural amino acids.
Article in Journal of the American Chemical Society, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 75 papers.
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Who cites it
75 citing papers in PubMed.
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- Control of Circularly Polarized Luminescence in Cholesteric Luminescent Liquid Crystals: From FRET to Exciton Coupling.Angewandte Chemie (International ed. in English) · 2026Article
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- Lipopolysaccharide confinement in the bacterial outer membrane is governed by interactions within the conserved Lipid A anchor.The EMBO journal · 2026Article
- Harnessing Frémy's salt for tyrosine-directed bioconjugations.RSC advances · 2026Article
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- Photostability of organic fluorophore influenced by adjacent amino acid residues.Communications chemistry · 2025Article
- Thioether editing generally increases the photostability of rhodamine dyes on self-labeling tags.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Tyrosine-Mediated Static and Dynamic Quenching of a Receptor Tyrosine Kinase Biosensor Reveals Inhibitor Binding Modes and Kinase Conformations.ACS chemical biology · 2025Article
- Direct observation of long-lived radical pair between flavin and guanine in single- and double-stranded DNA-oligomers.Communications chemistry · 2025Article
- The RNA Chaperone Hfq and Small Non-Coding RNAs Modulate the Biofilm Formation of the Fish PathogenInternational journal of molecular sciences · 2025Article
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- Voltage-clamp fluorometry for advancing mechanistic understanding of ion channel mechanisms with a focus on acid-sensing ion channels.Biochemical Society transactions · 2024Review
- Self-Responsive Fluorescence Aptasensor for Lactoferrin Determination in Dairy Products.Molecules (Basel, Switzerland) · 2024Article
- Specific, sensitive and quantitative protein detection by in-gel fluorescence.Nature communications · 2023Article
- Practical Guidance for Developing Small-Molecule Optical Probes for In Vivo Imaging.Molecular imaging and biology · 2023Review
- Exclusion of Anchor-Matched Peptide Nucleic Acid from Liquid-Ordered Domains by Hybridization with Complementary Flavin-Labeled DNA.ACS omega · 2023Article
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- Multiple mechanisms contribute to fluorometry signals from the voltage-gated proton channel.Communications biology · 2022Article
15 more citing papers are in PubMed but not listed here.
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5 authors.
Funding
Abstract
Quenching of fluorophores by the same proteins that they covalently label is a phenomenon that is neither well-known nor well-characterized. It is often assumed that fluorophores are unperturbed by their target proteins. However, it has been observed that attached fluorophores can be quenched by contact with amino acids within the same protein, and this property has been exploited to report on changing conformational states or intramolecular dynamics of proteins. We show in this communication that fluorescence of Alexa dyes is, in fact, quenched by interactions with Trp, Tyr, Met, and His residues through a combination of static and dynamic quenching mechanisms. In light of this finding, the potential effect of intramolecular quenching should be considered in the interpretation of data that involves quantitative measurements of fluorescence intensity in proteins.
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