ArticleVeterinary medicine international2010
Diagnostic Application of IS900 PCR Using Blood as a Source Sample for the Detection of Mycobacterium avium Subspecies Paratuberculosis in Early and Subclinical Cases of Caprine Paratuberculosis.
Article in Veterinary medicine international, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
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Who cites it
13 citing papers in PubMed, 37 citations in OpenAlex.
- Efficacy of 'indigenous vaccine' using native 'Indian bison type' genotype of Mycobacterium avium subspecies paratuberculosis for the control of clinical Johne's disease in an organized goat herd.Veterinary research communications · 2013Trial
- Comparative diagnostic evaluation and molecular typing of Mycobacterium avium subsp. Paratuberculosis in dromedary camels of Rajasthan, India.Veterinary research communications · 2025Article
- IS1311 PCR-RFLP molecular epidemiological approach for genotyping ofIranian journal of veterinary research · 2025Article
- Evaluation of Immune Exhaustion and Co-Inhibitory Receptor Expression inPathogens (Basel, Switzerland) · 2024Article
- Active surveillance and associated epidemiological risk factors of caprine paratuberculosis in selected district of Odisha, India.Veterinary research forum : an international quarterly journal · 2024Article
- Therapeutic management of Mycobacterium avium subspecies paratuberculosis infection with complete resolution of symptoms and disease in a patient with advanced inflammatory bowel syndrome.Molecular biology reports · 2021Article
- Using Omics Approaches in the Discovery of Biomarkers for Early Diagnosis of Johne's Disease in Sheep and Goats.Animals : an open access journal from MDPI · 2021Review
- Genetic Diversity AmongFrontiers in microbiology · 2020Article
- Comparison of multiplex and ordinary PCR for diagnosis of paratuberculosis and tuberculosis in blood samples (buffy coat) of cattle and buffaloes.Iranian journal of veterinary research · 2020Article
- Development and evaluation of LAMP-coupled lateral flow device for the detection of MAP in livestock at point of care resource-limited areas.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2019Article
- Comparative Evaluation of Different Test Combinations for Diagnosis of Mycobacterium avium Subspecies paratuberculosis Infecting Dairy Herds in India.BioMed research international · 2015Article
- A polymerase chain reaction and enzyme linked immunosorbent assay based approach for diagnosis and differentiation between vaccinated and infected cattle with Mycobacterium bovis.Journal of pharmacy & bioallied sciences · 2014Article
- Diagnosis of paratuberculosis in goats by cell mediated immune response, conventional and molecular diagnostic techniques.Tropical animal health and production · 2012Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Efficacy of IS900 blood PCR was evaluated for the presence of MAP infection. Serum, fecal, and blood samples of kids, young, and adult goats from farm and farmer's herds in Mathura district were also screened by ELISA, microscopy and culture. Of 111 goats (kids: 40, young: 14, adults: 57) screened, 77.5% were positive by blood PCR. Of 76 goats, 90.8% (kids: 87.5% and adults: 94.4%) were positive by PCR. From 21 kids and 14 young goats, 42.8 and 57.1% were positive. gDNA from goats was genotyped as MAP "Indian Bison type". Of 21 fecal samples of kids examined by microscopy, 66.7% were positive. In ELISA, 9.5 and 57.1% kids were positives as "type I" and "type II" reactors, respectively. Screening 14 young goats by culture of blood clots, 28.6% were positive. Agreement was substantial between PCR and microscopy. It was fair and moderate when PCR and microscopy were compared with type I and type II reactors, respectively. Presence of MAP in non-clinical kids and young goats indicate early or subclinical infection. Blood PCR was rapid, sensitive, and specific assay for detection of MAP in any stage (early, subclinical, and clinical) and age (kids, young, and adult) of goats.
Identifiers
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Registered trials
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