ArticleMolecular biology of the cell2010
Fusel alcohols regulate translation initiation by inhibiting eIF2B to reduce ternary complex in a mechanism that may involve altering the integrity and dynamics of the eIF2B body.
Article in Molecular biology of the cell, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed, 51 citations in OpenAlex.
- Fungistatic effect of sorbic acid on yeast cells via translational repression involving eIF2Microbial cell (Graz, Austria) · 2026Article
- eIF2B localization and its regulation during the integrated stress response is cell-type specific.iScience · 2024Article
- Paralogous translation factors target distinct mRNAs to differentially regulate tolerance to oxidative stress in yeast.Nucleic acids research · 2023Article
- Regulation and function of elF2B in neurological and metabolic disorders.Bioscience reports · 2022Review
- Overlapping regions of Caf20 mediate its interactions with the mRNA-5'cap-binding protein eIF4E and with ribosomes.Scientific reports · 2021Article
- Filament formation by the translation factor eIF2B regulates protein synthesis in starved cells.Biology open · 2020Article
- Reorganization of budding yeast cytoplasm upon energy depletion.Molecular biology of the cell · 2020Article
- Translation factor mRNA granules direct protein synthetic capacity to regions of polarized growth.The Journal of cell biology · 2019Article
- Cellular eIF2B subunit localization: implications for the integrated stress response and its control by small molecule drugs.Molecular biology of the cell · 2019Article
- Translational regulation in response to stress in Saccharomyces cerevisiae.Yeast (Chichester, England) · 2019Review
- Analysis of eIF2B bodies and their relationships with stress granules and P-bodies.Scientific reports · 2018Article
- Evolutionary engineering improves tolerance for medium-chain alcohols inBiotechnology for biofuels · 2018Article
- Dynamic changes in eIF4F-mRNA interactions revealed by global analyses of environmental stress responses.Genome biology · 2017Article
- Farnesol inhibits translation to limit growth and filamentation inMicrobial cell (Graz, Austria) · 2017Article
- Fail-safe control of translation initiation by dissociation of eIF2α phosphorylated ternary complexes.eLife · 2017Article
- Review
- Branched-Chain Aminotransferases Control TORC1 Signaling in Saccharomyces cerevisiae.PLoS genetics · 2015Article
- Integrated multi-omics analyses reveal the pleiotropic nature of the control of gene expression by Puf3p.Scientific reports · 2015Article
- The 4E-BP Caf20p Mediates Both eIF4E-Dependent and Independent Repression of Translation.PLoS genetics · 2015Article
- Alcohols inhibit translation to regulate morphogenesis in C. albicans.Fungal genetics and biology : FG & B · 2015Article
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Authors and funding
10 authors at 3 institutions in 1 country.
Funding
Abstract
Recycling of eIF2-GDP to the GTP-bound form constitutes a core essential, regulated step in eukaryotic translation. This reaction is mediated by eIF2B, a heteropentameric factor with important links to human disease. eIF2 in the GTP-bound form binds to methionyl initiator tRNA to form a ternary complex, and the levels of this ternary complex can be a critical determinant of the rate of protein synthesis. Here we show that eIF2B serves as the target for translation inhibition by various fusel alcohols in yeast. Fusel alcohols are endpoint metabolites from amino acid catabolism, which signal nitrogen scarcity. We show that the inhibition of eIF2B leads to reduced ternary complex levels and that different eIF2B subunit mutants alter fusel alcohol sensitivity. A DNA tiling array strategy was developed that overcame difficulties in the identification of these mutants where the phenotypic distinctions were too subtle for classical complementation cloning. Fusel alcohols also lead to eIF2alpha dephosphorylation in a Sit4p-dependent manner. In yeast, eIF2B occupies a large cytoplasmic body where guanine nucleotide exchange on eIF2 can occur and be regulated. Fusel alcohols impact on both the movement and dynamics of this 2B body. Overall, these results confirm that the guanine nucleotide exchange factor, eIF2B, is targeted by fusel alcohols. Moreover, they highlight a potential connection between the movement or integrity of the 2B body and eIF2B regulation.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.