ArticleNucleic acids research2010
tRNASec is transcribed by RNA polymerase II in Trypanosoma brucei but not in humans.
Article in Nucleic acids research, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 23 citations in OpenAlex.
- Genomic Organization of Trypanosoma cruzi tRNA Genes.Genome biology and evolution · 2025Article
- Historical Roles of Selenium and Selenoproteins in Health and Development: The Good, the Bad and the Ugly.International journal of molecular sciences · 2021Review
- Regulatory networking of the three RNA polymerases helps the eukaryotic cells cope with environmental stress.Current genetics · 2021Review
- Transfer RNAs: diversity in form and function.RNA biology · 2021Review
- The single CCA-adding enzyme ofThe Journal of biological chemistry · 2020Article
- A stable tRNA-like molecule is generated from the long noncoding RNA GUT15 in Arabidopsis.RNA biology · 2018Article
- A Multipurpose Toolkit to Enable Advanced Genome Engineering in Plants.The Plant cell · 2017Article
- The essential function of the Trypanosoma brucei Trl1 homolog in procyclic cells is maturation of the intron-containing tRNATyr.RNA (New York, N.Y.) · 2016Article
- The selenocysteine tRNA gene in leishmania major is transcribed by both RNA polymerase II and RNA polymerase III.Eukaryotic cell · 2015Article
- Transcriptional control of an essential ribozyme in Drosophila reveals an ancient evolutionary divide in animals.PLoS genetics · 2015Article
- Characterization of MRP RNA-protein interactions within the perinucleolar compartment.Molecular biology of the cell · 2011Article
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Authors and funding
7 authors at 2 institutions in 2 countries.
Funding
Abstract
Nuclear-encoded tRNAs are universally transcribed by RNA polymerase III (Pol-III) and contain intragenic promoters. Transcription of vertebrate tRNA(Sec) however requires extragenic promoters similar to Pol-III transcribed U6 snRNA. Here, we present a comparative analysis of tRNA(Sec) transcription in humans and the parasitic protozoa Trypanosoma brucei, two evolutionary highly diverged eukaryotes. RNAi-mediated ablation of Pol-II and Pol-III as well as oligo-dT induced transcription termination show that the human tRNA(Sec) is a Pol-III transcript. In T. brucei protein-coding genes are polycistronically transcribed by Pol-II and processed by trans-splicing and polyadenylation. tRNA genes are generally clustered in between polycistrons. However, the trypanosomal tRNA(Sec) genes are embedded within a polycistron. Their transcription is sensitive to α-amanitin and RNAi-mediated ablation of Pol-II, but not of Pol-III. Ectopic expression of the tRNA(Sec) outside but not inside a polycistron requires an added external promoter. These experiments demonstrate that trypanosomal tRNA(Sec), in contrast to its human counterpart, is transcribed by Pol-II. Synteny analysis shows that in trypanosomatids the tRNA(Sec) gene can be found in two different polycistrons, suggesting that it has evolved twice independently. Moreover, intron-encoded tRNAs are present in a number of eukaryotic genomes indicating that Pol-II transcription of tRNAs may not be restricted to trypanosomatids.
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