ReviewTrends in cell biology2010
Making the message clear: visualizing mRNA localization.
Review in Trends in cell biology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 50 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
50 citing papers in PubMed, 103 citations in OpenAlex.
- Step-by-Step Protocol for In Situ Profiling of RNA Subcellular Localization Using TATA-seq.Bio-protocol · 2026Article
- Imaging Spatiotemporal RNA and Protein Dynamics in Mouse Embryos.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Profiling RNA subcellular localization in situ by TATA-seq.RNA (New York, N.Y.) · 2025Article
- Boosting the toolbox for live imaging of translation.RNA (New York, N.Y.) · 2024Article
- Subcellular RNA distribution and its change during human embryonic stem cell differentiation.Stem cell reports · 2024Article
- High-Throughput RNA-HCR-FISH Detection of Endogenous Pre-mRNA Splice Variants.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Condensate functionalization with microtubule motors directs their nucleation in space and allows manipulating RNA localization.The EMBO journal · 2023Article
- Far from home: the role of glial mRNA localization in synaptic plasticity.RNA (New York, N.Y.) · 2023Review
- GM-lncLoc: LncRNAs subcellular localization prediction based on graph neural network with meta-learning.BMC genomics · 2023Article
- LAFITE Reveals the Complexity of Transcript Isoforms in Subcellular Fractions.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2023Article
- Review
- Inert Pepper aptamer-mediated endogenous mRNA recognition and imaging in living cells.Nucleic acids research · 2022Article
- Subcellular Transcriptomics and Proteomics: A Comparative Methods Review.Molecular & cellular proteomics : MCP · 2022Review
- mLoc-mRNA: predicting multiple sub-cellular localization of mRNAs using random forest algorithm coupled with feature selection via elastic net.BMC bioinformatics · 2021Article
- Human nucleoli comprise multiple constrained territories, tethered to individual chromosomes.Genes & development · 2021Article
- New Generations of MS2 Variants and MCP Fusions to Detect Single mRNAs in Living Eukaryotic Cells.Methods in molecular biology (Clifton, N.J.) · 2020Article
- Article
- mRNA Detection with Fluorescence-base Imaging Techniques for Arthritis Diagnosis.Journal of rheumatology research · 2019Article
- Article
- Targeted Imaging of VCAM-1 mRNA in a Mouse Model of Laser-Induced Choroidal Neovascularization Using Antisense Hairpin-DNA-Functionalized Gold-Nanoparticles.Molecular pharmaceutics · 2018Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Localized mRNA provides spatial and temporal protein expression essential to cell development and physiology. To explore the mechanisms involved, considerable effort has been spent in establishing new and improved methods for visualizing mRNA. Here, we discuss how these techniques have extended our understanding of intracellular mRNA localization in a variety of organisms. In addition to increased ease and specificity of detection in fixed tissue, in situ hybridization methods now enable examination of mRNA distribution at the ultrastructural level with electron microscopy. Most significantly, methods for following the movement of mRNA in living cells are now in widespread use. These include the introduction of labeled transcripts by microinjection, hybridization based methods using labeled antisense probes and complementary transgenic methods for tagging endogenous mRNAs using bacteriophage components. These technical innovations are now being coupled with super-resolution light microscopy methods and promise to revolutionize our understanding of the dynamics and complexity of the molecular mechanism of mRNA localization.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.