Evidence map›Paper›PMID 20444245›Full record

ArticleMobile DNA2010

Two-hybrid analysis of Ty3 capsid subdomain interactions.

Min Zhang, Liza Sz Larsen, Becky Irwin, Virginia Bilanchone, Suzanne Sandmeyer

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Mobile DNA, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
0.1field-weighted citation impact, top 47% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 5 citations in OpenAlex.

  1. Review
  2. Article
  3. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 1 institution in 1 country.

Min Zhang *Department of Biological Chemistry, University of California, Irvine, CA, USA.
Liza Sz Larsen *Department of Biological Chemistry, University of California, Irvine, CA, USA.
Becky IrwinDepartment of Biological Chemistry, University of California, Irvine, CA, USA.
Virginia BilanchoneDepartment of Biological Chemistry, University of California, Irvine, CA, USA.
Suzanne SandmeyerDepartment of Biological Chemistry, University of California, Irvine, CA, USA.
University of California, Irvine · US

Funding

Ty3 Viruslike Particle Morphogenesis and Host InteractionsR01GM033281 · NIGMS · UNIVERSITY OF CALIFORNIA-IRVINE · PI SANDMEYER, SUZANNE · 1985 to 2013
$4.7M
NIGMS NIH HHS R01 GM033281
6 · The paper itself

Abstract

backgroundThe yeast retrotransposon Ty3 forms stable virus-like particles. Gag3, the major structural protein, is composed of capsid, spacer and nucleocapsid domains. The capsid domain of Gag3 was previously modeled as a structure similar to retrovirus capsid.

findingsTwo-hybrid analysis was used to understand the interactions that contribute to particle assembly. Gag3 interacted with itself as predicted based on its role as the major structural protein. The N-terminal subdomain (NTD) of the capsid was able to interact with itself and with the C-terminal subdomain (CTD) of the capsid, but interacted less well with intact Gag3. Mutations previously shown to block particle assembly disrupted Gag3 interactions more than subdomain interactions.

conclusionsThe findings that the NTD interacts with itself and with the CTD are consistent with previous modeling and a role similar to that of the capsid in retrovirus particle structure. These results are consistent with a model in which the Gag3-Gag3 interactions that initiate assembly differ from the subdomain interactions that potentially underlie particle stability.

Identifiers

PMID20444245
PMCPMC2878294
OpenAlexW2031211029

What OpenQuestion holds

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LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.