ArticleMolecular microbiology2010
Cell wall synthesis is necessary for membrane dynamics during sporulation of Bacillus subtilis.
Article in Molecular microbiology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 42 papers.
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Who cites it
42 citing papers in PubMed, 70 citations in OpenAlex.
- A safeguarding factor monitors septal pore stability to secure compartmentalization during sporulation in Bacillus subtilis.Communications biology · 2026Article
- Modulation of the Sporulation Dynamics in the Plant-Probiotic Bacillus velezensis 83 via Carbon and Quorum-Sensing Metabolites.Probiotics and antimicrobial proteins · 2025Article
- A new fluorescence-based approach for direct visualization of coat formation during sporulation in Bacillus cereus.Scientific reports · 2023Article
- Article
- FisB relies on homo-oligomerization and lipid binding to catalyze membrane fission in bacteria.PLoS biology · 2021Article
- Shaping an Endospore: Architectural Transformations DuringAnnual review of microbiology · 2020Review
- Inducible intracellular membranes: molecular aspects and emerging applications.Microbial cell factories · 2020Review
- Article
- Sporulation and Germination in Clostridial Pathogens.Microbiology spectrum · 2019Review
- The molecular architecture of engulfment duringeLife · 2019Article
- Peptidoglycan degradation machinery in Clostridium difficile forespore engulfment.Molecular microbiology · 2018Article
- Differential requirements for conserved peptidoglycan remodeling enzymes during Clostridioides difficile spore formation.Molecular microbiology · 2018Article
- Resuscitation-Promoting Factors Are Required for Mycobacterium smegmatis Biofilm Formation.Applied and environmental microbiology · 2018Article
- Autoregulation of SafA Assembly through Recruitment of a Protein Cross-Linking Enzyme.Journal of bacteriology · 2018Article
- Article
- Hydrolysis of peptidoglycan is modulated by amidation of meso-diaminopimelic acid and MgMolecular microbiology · 2017Article
- Disruption of Two-component System LytSR Affects Forespore Engulfment inFrontiers in cellular and infection microbiology · 2017Article
- Cell-wall remodeling drives engulfment duringeLife · 2016Article
- Membrane remodelling in bacteria.Journal of structural biology · 2016Review
- Sporulation, bacterial cell envelopes and the origin of life.Nature reviews. Microbiology · 2016Review
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
During Bacillus subtilis sporulation, an endocytic-like process called engulfment results in one cell being entirely encased in the cytoplasm of another cell. The driving force underlying this process of membrane movement has remained unclear, although components of the machinery have been characterized. Here we provide evidence that synthesis of peptidoglycan, the rigid, strength bearing extracellular polymer of bacteria, is a key part of the missing force-generating mechanism for engulfment. We observed that sites of peptidoglycan synthesis initially coincide with the engulfing membrane and later with the site of engulfment membrane fission. Furthermore, compounds that block muropeptide synthesis or polymerization prevented membrane migration in cells lacking a component of the engulfment machinery (SpoIIQ), and blocked the membrane fission event at the completion of engulfment in all cells. In addition, these compounds inhibited bulge and vesicle formation that occur in spoIID mutant cells unable to initiate engulfment, as did genetic ablation of a protein that polymerizes muropeptides. This is the first report to our knowledge that peptidoglycan synthesis is necessary for membrane movements in bacterial cells and has implications for the mechanism of force generation during cytokinesis.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.